Detailed information of TA system TA200792

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 51590..51859
• Replicon: plasmid pYLMB49a
• Accession: NZ_CP073954
• Organism: Escherichia coli strain MB49

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KFU90_RS23490
• Protein ID: WP_001372321.1
• Coordinates: 51734..51859 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	51590..51655 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU90_RS23445	46642..46872	+	231	WP_072179139.1	hypothetical protein	-
KFU90_RS23450	47113..47319	+	207	WP_000547971.1	hypothetical protein	-
KFU90_RS23455	47345..47884	+	540	WP_009426937.1	single-stranded DNA-binding protein	-
KFU90_RS23460	47940..48173	+	234	WP_000006030.1	DUF905 family protein	-
KFU90_RS23465	48238..50202	+	1965	WP_257188505.1	ParB/RepB/Spo0J family partition protein	-
KFU90_RS23470	50271..50705	+	435	WP_000845894.1	conjugation system SOS inhibitor PsiB	-
KFU90_RS23475	50702..51464	+	763	Protein_64	plasmid SOS inhibition protein A	-
KFU90_RS23480	51433..51621	-	189	WP_001299721.1	hypothetical protein	-
-	51433..51657	+	225	NuclAT_0	-	-
-	51433..51657	+	225	NuclAT_0	-	-
-	51433..51657	+	225	NuclAT_0	-	-
-	51433..51657	+	225	NuclAT_0	-	-
-	51590..51655	-	66	-	-	Antitoxin
KFU90_RS23485	51643..51792	+	150	Protein_66	plasmid maintenance protein Mok	-
KFU90_RS23490	51734..51859	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KFU90_RS23495	52079..52309	+	231	WP_071586998.1	hypothetical protein	-
KFU90_RS23500	52307..52479	-	173	Protein_69	hypothetical protein	-
KFU90_RS23505	52549..52755	+	207	WP_000547968.1	hypothetical protein	-
KFU90_RS23510	52780..53067	+	288	WP_000107535.1	hypothetical protein	-
KFU90_RS23515	53185..54006	+	822	WP_001234502.1	DUF932 domain-containing protein	-
KFU90_RS23520	54285..54737	-	453	WP_001469955.1	transglycosylase SLT domain-containing protein	-
KFU90_RS23525	55250..55645	+	396	WP_089581130.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KFU90_RS23530	55806..56570	+	765	WP_089581129.1	hypothetical protein	-
KFU90_RS23535	56710..56859	+	150	WP_001401982.1	TraY domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / aph(3'')-Ib / aph(6)-Id	-	1..94480	94480

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T200792 WP_001372321.1 NZ_CP073954:51734-51859 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T200792 NZ_CP098175:c4813786-4813439 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT200792 NZ_CP073954:c51655-51590 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin