Detailed information of TA system TA200791

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4629141..4629743
• Replicon: chromosome
• Accession: NZ_CP073953
• Organism: Escherichia coli strain MB49

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KFU90_RS22170
• Protein ID: WP_000897305.1
• Coordinates: 4629432..4629743 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KFU90_RS22165
• Protein ID: WP_000356397.1
• Coordinates: 4629141..4629431 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU90_RS22140 (4625066)	4625066..4625968	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KFU90_RS22145 (4625965)	4625965..4626600	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KFU90_RS22150 (4626597)	4626597..4627526	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KFU90_RS22155 (4627856)	4627856..4628098	-	243	WP_001087409.1	protein YiiF	-
KFU90_RS22160 (4628318)	4628318..4628536	-	219	WP_001315930.1	CopG family transcriptional regulator	-
KFU90_RS22165 (4629141)	4629141..4629431	-	291	WP_000356397.1	NadS family protein	Antitoxin
KFU90_RS22170 (4629432)	4629432..4629743	-	312	WP_000897305.1	hypothetical protein	Toxin
KFU90_RS22175 (4629972)	4629972..4630880	+	909	WP_001162704.1	alpha/beta hydrolase	-
KFU90_RS22180 (4630944)	4630944..4631885	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KFU90_RS22185 (4631930)	4631930..4632367	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KFU90_RS22190 (4632364)	4632364..4633236	-	873	WP_000920762.1	virulence factor BrkB family protein	-
KFU90_RS22195 (4633230)	4633230..4633829	-	600	WP_001295269.1	glucose-1-phosphatase	-
KFU90_RS22200 (4633928)	4633928..4634713	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T200791 WP_000897305.1 NZ_CP073953:c4629743-4629432 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T200791 NZ_CP098175:c4382262-4381852 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT200791 WP_000356397.1 NZ_CP073953:c4629431-4629141 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT200791 NZ_CP098175:c4382509-4382243 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7