Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3741476..3742017 Replicon chromosome
Accession NZ_CP073953
Organism Escherichia coli strain MB49

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag KFU90_RS18095 Protein ID WP_000615976.1
Coordinates 3741739..3742017 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KFU90_RS18090 Protein ID WP_000729704.1
Coordinates 3741476..3741736 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU90_RS18065 (3736512) 3736512..3737651 + 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -
KFU90_RS18070 (3737635) 3737635..3739728 + 2094 WP_128995100.1 flagellar biosynthesis protein FlhA -
KFU90_RS18075 (3739833) 3739833..3740111 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
KFU90_RS18080 (3740104) 3740104..3740460 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
KFU90_RS18085 (3740517) 3740517..3741290 - 774 WP_000543897.1 C40 family peptidase -
KFU90_RS18090 (3741476) 3741476..3741736 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KFU90_RS18095 (3741739) 3741739..3742017 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KFU90_RS18100 (3742173) 3742173..3742913 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KFU90_RS18105 (3742884) 3742884..3743651 - 768 WP_000333380.1 class II glutamine amidotransferase -
KFU90_RS18110 (3743857) 3743857..3744435 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T200786 WP_000615976.1 NZ_CP073953:3741739-3742017 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T200786 NZ_CP098175:522950-523234 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT200786 WP_000729704.1 NZ_CP073953:3741476-3741736 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT200786 NZ_CP098175:522718-522960 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References