Detailed information of TA system TA020077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/-
• Location: 1978164..1978666
• Replicon: chromosome
• Accession: HG738867
• Organism: Escherichia coli str. K-12 substr. MC4100

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: BN896_1807
• Protein ID: CDJ72387.1
• Coordinates: 1978164..1978418 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: BN896_1808
• Protein ID: CDJ72388.1
• Coordinates: 1978415..1978666 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN896_1802	1973179..1973406	-	228	CDJ72382.1	hypothetical protein	-
BN896_1803	1973420..1974478	-	1059	CDJ72383.1	putative inner membrane protein	-
BN896_1804	1974657..1976015	-	1359	CDJ72384.1	putative amino-acid transporter	-
BN896_1805	1976282..1977211	-	930	CDJ72385.1	putative DNA-binding transcriptional regulator	-
BN896_1806	1977257..1978081	-	825	CDJ72386.1	putative epimerase, with NAD(P)-binding Rossmann-fold domain	-
BN896_1807	1978164..1978418	-	255	CDJ72387.1	toxin of the YoeB-YefM toxin-antitoxin system	Toxin
BN896_1808	1978415..1978666	-	252	CDJ72388.1	antitoxin YefM	Antitoxin
BN896_1809	1978949..1978999	+	51	CDJ72389.1	his operon leader peptide	-
BN896_1810	1979145..1980044	+	900	CDJ72390.1	ATP phosphoribosyltransferase	-
BN896_1811	1980050..1981354	+	1305	CDJ72391.1	histidinol dehydrogenase	-
BN896_1812	1981351..1982421	+	1071	CDJ72392.1	histidinol-phosphate aminotransferase	-
BN896_1813	1982421..1983488	+	1068	CDJ72393.1	imidazole glycerol-phosphate dehydratase/histidinol phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T20077 CDJ72387.1 HG738867:c1978418-1978164 [Escherichia coli str. K-12 substr. MC4100]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T20077 HG738867:c1978418-1978164 [Escherichia coli str. K-12 substr. MC4100]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT20077 CDJ72388.1 HG738867:c1978666-1978415 [Escherichia coli str. K-12 substr. MC4100]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT20077 HG738867:c1978666-1978415 [Escherichia coli str. K-12 substr. MC4100]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA