Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3665029..3665570 Replicon chromosome
Accession NZ_CP073949
Organism Escherichia coli strain MB50

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag KFU75_RS17935 Protein ID WP_000615979.1
Coordinates 3665292..3665570 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KFU75_RS17930 Protein ID WP_000729704.1
Coordinates 3665029..3665289 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU75_RS17910 (3660712) 3660712..3661497 - 786 WP_000207549.1 putative lateral flagellar export/assembly protein LafU -
KFU75_RS17915 (3661469) 3661469..3663181 + 1713 Protein_3507 flagellar biosynthesis protein FlhA -
KFU75_RS17920 (3663397) 3663397..3663894 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
KFU75_RS17925 (3664070) 3664070..3664843 - 774 WP_001353765.1 C40 family peptidase -
KFU75_RS17930 (3665029) 3665029..3665289 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KFU75_RS17935 (3665292) 3665292..3665570 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KFU75_RS17940 (3665726) 3665726..3666466 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KFU75_RS17945 (3666437) 3666437..3667204 - 768 WP_257186918.1 class II glutamine amidotransferase -
KFU75_RS17950 (3667410) 3667410..3667988 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3640806..3676183 35377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T200764 WP_000615979.1 NZ_CP073949:3665292-3665570 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T200764 NZ_CP098167:c4733914-4733573 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGTCTGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAAGGGATTC
AACTGGCAAGAACACTCCCCATATCTGCGGGCAGTTGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGCGCCGAAG
CCGTAAAAATGCTGTACGTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT200764 WP_000729704.1 NZ_CP073949:3665029-3665289 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT200764 NZ_CP098167:c4734276-4733935 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTTCACTATCTGGCTGACCGGGCCAGCATTACCGGTGAATTCAGCAACGCAGAATCTT
TGAAGTTGGATGAGGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTTTTTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References