Detailed information of TA system TA200738

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3755340..3756034
• Replicon: chromosome
• Accession: NZ_CP073946
• Organism: Escherichia coli strain MB51

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: KFU68_RS18425
• Protein ID: WP_001263493.1
• Coordinates: 3755340..3755738 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: KFU68_RS18430
• Protein ID: WP_000554757.1
• Coordinates: 3755741..3756034 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3751000)	3751000..3751080	-	81	NuclAT_12	-	-
- (3751000)	3751000..3751080	-	81	NuclAT_12	-	-
- (3751000)	3751000..3751080	-	81	NuclAT_12	-	-
- (3751000)	3751000..3751080	-	81	NuclAT_12	-	-
KFU68_RS18395 (3750340)	3750340..3751584	-	1245	WP_000189541.1	esterase FrsA	-
KFU68_RS18400 (3751676)	3751676..3752134	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KFU68_RS18405 (3752395)	3752395..3753852	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
KFU68_RS18410 (3753909)	3753909..3754430	-	522	Protein_3603	peptide chain release factor H	-
KFU68_RS18415 (3754429)	3754429..3754632	-	204	Protein_3604	RtcB family protein	-
KFU68_RS18420 (3754878)	3754878..3755330	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
KFU68_RS18425 (3755340)	3755340..3755738	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KFU68_RS18430 (3755741)	3755741..3756034	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KFU68_RS18435 (3756086)	3756086..3757141	-	1056	WP_001226164.1	DNA polymerase IV	-
KFU68_RS18440 (3757212)	3757212..3757997	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
KFU68_RS18445 (3757969)	3757969..3759681	+	1713	Protein_3610	flagellar biosynthesis protein FlhA	-
KFU68_RS18450 (3759905)	3759905..3760402	-	498	Protein_3611	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	3736474..3786775	50301

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T200738 WP_001263493.1 NZ_CP073946:c3755738-3755340 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T200738 NZ_CP098161:c3343024-3342922 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT200738 WP_000554757.1 NZ_CP073946:c3756034-3755741 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT200738 NZ_CP098161:3342915-3343060 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6