Detailed information of TA system TA200733

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2621042..2621413
• Replicon: chromosome
• Accession: NZ_CP073946
• Organism: Escherichia coli strain MB51

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: KFU68_RS12835
• Protein ID: WP_001317028.1
• Coordinates: 2621219..2621413 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2621042..2621220 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU68_RS12810 (2616997)	2616997..2618370	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
KFU68_RS12815 (2618499)	2618499..2619434	-	936	WP_001157406.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
KFU68_RS12820 (2619486)	2619486..2620721	-	1236	WP_000040858.1	site-specific integrase	-
KFU68_RS12825 (2620723)	2620723..2620938	-	216	WP_000079604.1	excisionase XisR	-
- (2621042)	2621042..2621220	+	179	NuclAT_0	-	Antitoxin
- (2621042)	2621042..2621220	+	179	NuclAT_0	-	Antitoxin
- (2621042)	2621042..2621220	+	179	NuclAT_0	-	Antitoxin
- (2621042)	2621042..2621220	+	179	NuclAT_0	-	Antitoxin
KFU68_RS12830 (2621017)	2621017..2621226	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
KFU68_RS12835 (2621219)	2621219..2621413	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
KFU68_RS12845 (2622278)	2622278..2623210	+	933	WP_000583176.1	sensor domain-containing diguanylate cyclase	-
KFU68_RS12850 (2623231)	2623231..2623794	-	564	WP_001046829.1	DNA endonuclease SmrA	-
KFU68_RS12855 (2624124)	2624124..2625032	-	909	WP_000885454.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2583329..2639155	55826

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T200733 WP_001317028.1 NZ_CP073946:c2621413-2621219 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T200733 NZ_CP098161:622114-622455 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGTCTGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAAGGGATTC
AACTGGCAAGAACACTCCCCATATCTGCGGGCAGTTGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGCGCCGAAG
CCGTAAAAATGCTGTACGTTAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT200733 NZ_CP073946:2621042-2621220 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin