Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 603506..604305 Replicon chromosome
Accession NZ_CP073946
Organism Escherichia coli strain MB51

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag KFU68_RS02920 Protein ID WP_000347273.1
Coordinates 603506..603970 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KFU68_RS02925 Protein ID WP_001307405.1
Coordinates 603970..604305 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU68_RS02890 (598507) 598507..598941 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KFU68_RS02895 (598959) 598959..599837 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KFU68_RS02900 (599827) 599827..600606 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
KFU68_RS02905 (600617) 600617..601090 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
KFU68_RS02910 (601113) 601113..602393 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
KFU68_RS02915 (602642) 602642..603451 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KFU68_RS02920 (603506) 603506..603970 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KFU68_RS02925 (603970) 603970..604305 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KFU68_RS02930 (604454) 604454..606025 - 1572 WP_001273753.1 galactarate dehydratase -
KFU68_RS02935 (606400) 606400..607734 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KFU68_RS02940 (607750) 607750..608520 + 771 WP_001058221.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T200718 WP_000347273.1 NZ_CP073946:c603970-603506 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T200718 NZ_CP098159:383795-384142 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT200718 WP_001307405.1 NZ_CP073946:c604305-603970 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT200718 NZ_CP098159:384142-384441 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References