Detailed information of TA system TA200714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3604154..3604848
• Replicon: chromosome
• Accession: NZ_CP073943
• Organism: Escherichia coli strain MB52

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: KFU89_RS17350
• Protein ID: WP_001263493.1
• Coordinates: 3604154..3604552 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: KFU89_RS17355
• Protein ID: WP_152927302.1
• Coordinates: 3604555..3604848 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3599212)	3599212..3599292	-	81	NuclAT_8	-	-
- (3599212)	3599212..3599292	-	81	NuclAT_8	-	-
- (3599212)	3599212..3599292	-	81	NuclAT_8	-	-
- (3599212)	3599212..3599292	-	81	NuclAT_8	-	-
KFU89_RS17320 (3599888)	3599888..3600346	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KFU89_RS17325 (3600607)	3600607..3602064	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
KFU89_RS17330 (3602121)	3602121..3602642	-	522	Protein_3388	peptide chain release factor H	-
KFU89_RS17335 (3602641)	3602641..3602844	-	204	Protein_3389	RtcB family protein	-
KFU89_RS17345 (3603854)	3603854..3604144	-	291	Protein_3391	GNAT family N-acetyltransferase	-
KFU89_RS17350 (3604154)	3604154..3604552	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KFU89_RS17355 (3604555)	3604555..3604848	-	294	WP_152927302.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KFU89_RS17360 (3604900)	3604900..3605955	-	1056	WP_001226164.1	DNA polymerase IV	-
KFU89_RS17365 (3606026)	3606026..3606811	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
KFU89_RS17370 (3606783)	3606783..3608495	+	1713	Protein_3396	flagellar biosynthesis protein FlhA	-
KFU89_RS17375 (3608719)	3608719..3609216	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3603102..3603605	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T200714 WP_001263493.1 NZ_CP073943:c3604552-3604154 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T200714 NZ_CP098149:c4795708-4795424 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11180.76 Da
• Isoelectric Point: 5.2168

>AT200714 WP_152927302.1 NZ_CP073943:c3604848-3604555 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKACFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT200714 NZ_CP098149:c4795940-4795698 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGGTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: S1EXB8