Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3580181..3580875 Replicon chromosome
Accession NZ_CP073939
Organism Escherichia coli strain MB53

Toxin (Protein)


Gene name yafO Uniprot ID A0A080JBV5
Locus tag KFU83_RS17480 Protein ID WP_032280310.1
Coordinates 3580181..3580579 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag KFU83_RS17485 Protein ID WP_000554758.1
Coordinates 3580582..3580875 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3575956) 3575956..3576036 - 81 NuclAT_13 - -
- (3575956) 3575956..3576036 - 81 NuclAT_13 - -
- (3575956) 3575956..3576036 - 81 NuclAT_13 - -
- (3575956) 3575956..3576036 - 81 NuclAT_13 - -
KFU83_RS17450 (3575296) 3575296..3576540 - 1245 WP_000189542.1 esterase FrsA -
KFU83_RS17455 (3576632) 3576632..3577090 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
KFU83_RS17460 (3577351) 3577351..3578808 + 1458 WP_001293010.1 cytosol nonspecific dipeptidase -
KFU83_RS17465 (3578865) 3578865..3579173 - 309 Protein_3420 peptide chain release factor H -
KFU83_RS17470 (3579193) 3579193..3579401 - 209 Protein_3421 RtcB family protein -
KFU83_RS17475 (3579719) 3579719..3580171 - 453 WP_001059871.1 GNAT family N-acetyltransferase -
KFU83_RS17480 (3580181) 3580181..3580579 - 399 WP_032280310.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
KFU83_RS17485 (3580582) 3580582..3580875 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
KFU83_RS17490 (3580927) 3580927..3581982 - 1056 WP_001226183.1 DNA polymerase IV -
KFU83_RS17495 (3582053) 3582053..3582838 - 786 WP_000207551.1 putative lateral flagellar export/assembly protein LafU -
KFU83_RS17500 (3582810) 3582810..3584522 + 1713 Protein_3427 flagellar biosynthesis protein FlhA -
KFU83_RS17505 (3584844) 3584844..3585593 - 750 WP_032280313.1 C40 family peptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15501.91 Da        Isoelectric Point: 8.0949

>T200683 WP_032280310.1 NZ_CP073939:c3580579-3580181 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTVDFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T200683 NZ_CP098141:1864074-1864176 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT200683 WP_000554758.1 NZ_CP073939:c3580875-3580582 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT200683 NZ_CP098141:c1864183-1864038 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A080JBV5

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References