Detailed information of TA system TA200664

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3731197..3731891
• Replicon: chromosome
• Accession: NZ_CP073936
• Organism: Escherichia coli strain MB56

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A3P5GWS5
• Locus tag: KFU61_RS18080
• Protein ID: WP_001263488.1
• Coordinates: 3731197..3731595 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: KFU61_RS18085
• Protein ID: WP_257181949.1
• Coordinates: 3731598..3731891 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3726785)	3726785..3726865	-	81	NuclAT_12	-	-
- (3726785)	3726785..3726865	-	81	NuclAT_12	-	-
- (3726785)	3726785..3726865	-	81	NuclAT_12	-	-
- (3726785)	3726785..3726865	-	81	NuclAT_12	-	-
KFU61_RS18055 (3727461)	3727461..3727919	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KFU61_RS18060 (3728180)	3728180..3729637	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
KFU61_RS18065 (3729694)	3729694..3730215	-	522	Protein_3529	peptide chain release factor H	-
KFU61_RS18070 (3730211)	3730211..3730417	-	207	Protein_3530	RtcB family protein	-
KFU61_RS18075 (3730735)	3730735..3731187	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
KFU61_RS18080 (3731197)	3731197..3731595	-	399	WP_001263488.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KFU61_RS18085 (3731598)	3731598..3731891	-	294	WP_257181949.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KFU61_RS18090 (3731943)	3731943..3732998	-	1056	WP_001226164.1	DNA polymerase IV	-
KFU61_RS18095 (3733069)	3733069..3733854	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
KFU61_RS18100 (3733826)	3733826..3735538	+	1713	Protein_3536	flagellar biosynthesis protein FlhA	-
KFU61_RS18105 (3735762)	3735762..3736259	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15559.90 Da
• Isoelectric Point: 6.9798

>T200664 WP_001263488.1 NZ_CP073936:c3731595-3731197 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQEAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T200664 NZ_CP098136:1888618-1888836 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11281.81 Da
• Isoelectric Point: 5.2170

>AT200664 WP_257181949.1 NZ_CP073936:c3731891-3731598 [Escherichia coli]
MHRILAEKSFNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT200664 NZ_CP098136:1888217-1888591 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A3P5GWS5