Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3596802..3597496 Replicon chromosome
Accession NZ_CP073932
Organism Escherichia coli strain MB57

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag KFU62_RS17355 Protein ID WP_001263489.1
Coordinates 3596802..3597200 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag KFU62_RS17360 Protein ID WP_000554758.1
Coordinates 3597203..3597496 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3592390) 3592390..3592470 - 81 NuclAT_12 - -
- (3592390) 3592390..3592470 - 81 NuclAT_12 - -
- (3592390) 3592390..3592470 - 81 NuclAT_12 - -
- (3592390) 3592390..3592470 - 81 NuclAT_12 - -
KFU62_RS17330 (3593066) 3593066..3593524 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
KFU62_RS17335 (3593785) 3593785..3595242 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
KFU62_RS17340 (3595299) 3595299..3595820 - 522 Protein_3388 peptide chain release factor H -
KFU62_RS17345 (3595816) 3595816..3596022 - 207 Protein_3389 RtcB family protein -
KFU62_RS17350 (3596340) 3596340..3596792 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
KFU62_RS17355 (3596802) 3596802..3597200 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
KFU62_RS17360 (3597203) 3597203..3597496 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
KFU62_RS17365 (3597548) 3597548..3598603 - 1056 WP_001226164.1 DNA polymerase IV -
KFU62_RS17370 (3598674) 3598674..3599459 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
KFU62_RS17375 (3599431) 3599431..3601143 + 1713 Protein_3395 flagellar biosynthesis protein FlhA -
KFU62_RS17380 (3601367) 3601367..3601864 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T200638 WP_001263489.1 NZ_CP073932:c3597200-3596802 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T200638 NZ_CP098128:c3317450-3317348 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT200638 WP_000554758.1 NZ_CP073932:c3597496-3597203 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT200638 NZ_CP098128:3317341-3317486 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References