Detailed information of TA system TA200621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 736473..737166
• Replicon: chromosome
• Accession: NZ_CP073932
• Organism: Escherichia coli strain MB57

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: KFU62_RS03595
• Protein ID: WP_000415584.1
• Coordinates: 736473..736769 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: KFU62_RS03600
• Protein ID: WP_000650107.1
• Coordinates: 736771..737166 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU62_RS03560 (731561)	731561..731875	-	315	WP_000958598.1	putative quinol monooxygenase	-
KFU62_RS03565 (731906)	731906..732487	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
KFU62_RS03570 (732806)	732806..733138	+	333	WP_000917685.1	DUF2645 family protein	-
KFU62_RS03575 (733184)	733184..734533	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
KFU62_RS03580 (734530)	734530..735189	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
KFU62_RS03585 (735341)	735341..735733	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
KFU62_RS03590 (735786)	735786..736268	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
KFU62_RS03595 (736473)	736473..736769	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
KFU62_RS03600 (736771)	736771..737166	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
KFU62_RS03605 (737299)	737299..738906	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
KFU62_RS03610 (739044)	739044..741302	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T200621 WP_000415584.1 NZ_CP073932:736473-736769 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T200621 NZ_CP098123:1531166-1531648 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT200621 WP_000650107.1 NZ_CP073932:736771-737166 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT200621 NZ_CP098123:1530912-1531178 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65