Detailed information of TA system TA200613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3763651..3764345
• Replicon: chromosome
• Accession: NZ_CP073929
• Organism: Escherichia coli strain MB64

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: KFU91_RS18110
• Protein ID: WP_001263493.1
• Coordinates: 3763651..3764049 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: KFU91_RS18115
• Protein ID: WP_000554757.1
• Coordinates: 3764052..3764345 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3759311)	3759311..3759391	-	81	NuclAT_9	-	-
- (3759311)	3759311..3759391	-	81	NuclAT_9	-	-
- (3759311)	3759311..3759391	-	81	NuclAT_9	-	-
- (3759311)	3759311..3759391	-	81	NuclAT_9	-	-
KFU91_RS18080 (3758651)	3758651..3759895	-	1245	WP_000189541.1	esterase FrsA	-
KFU91_RS18085 (3759987)	3759987..3760445	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KFU91_RS18090 (3760706)	3760706..3762163	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
KFU91_RS18095 (3762220)	3762220..3762741	-	522	Protein_3539	peptide chain release factor H	-
KFU91_RS18100 (3762740)	3762740..3762943	-	204	Protein_3540	RtcB family protein	-
KFU91_RS18105 (3763189)	3763189..3763641	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
KFU91_RS18110 (3763651)	3763651..3764049	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KFU91_RS18115 (3764052)	3764052..3764345	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KFU91_RS18120 (3764397)	3764397..3765452	-	1056	WP_001226164.1	DNA polymerase IV	-
KFU91_RS18125 (3765523)	3765523..3766308	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
KFU91_RS18130 (3766280)	3766280..3767992	+	1713	Protein_3546	flagellar biosynthesis protein FlhA	-
KFU91_RS18135 (3768216)	3768216..3768713	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T200613 WP_001263493.1 NZ_CP073929:c3764049-3763651 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T200613 NZ_CP098040:3368137-3368427 [Providencia rettgeri]
ATGACAGGGTTCTATAAAGTTAAATTTAGGGATGATGCAGCAAAAGAGTGGAAAAAGCTTGGTGCAACAATACAGAAGCA
ATTTGCTAAGAAACTAAAATTACTGATTGAGAACCCTCATATACCTTCTGCAAGGCTCAGTGGGCTGCAAAATTGTTATA
AGATTAAGCTGAAAGCATCAGGTTATCGCTTAGTATACGAAGTTGTTGATAATCAGCTTATTATAATTGTGGTCACTGTG
GGGAAAAGGAATCGTAACGAGGTTTATGATATTGCAAAAAAGCGGCTATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT200613 WP_000554757.1 NZ_CP073929:c3764345-3764052 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT200613 NZ_CP098040:3367889-3368140 [Providencia rettgeri]
ATGCCTCAAGTTATTTTAAGTGATGTTACGGCGAGCATCAGTGAACTAAAGAAAAACCCTATGGCGACAGTGGATTCTGG
TGAGGGGTTCCCTGTGGCTATTTTGAATAGAAATAAGACTGCATTTTACTGTGTTCCAGCTGAAATATATGAAAATTTAT
TAGAACAAATTGAAGACAAAGAACTTATTGAAATTGTTAACGCAAGGAAAAACCAGAAGCTTGTAGATGTGGATTTGGAT
AGCTATTTATGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6