Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 681074..681801 Replicon chromosome
Accession NZ_CP073929
Organism Escherichia coli strain MB64

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KFU91_RS03310 Protein ID WP_000550189.1
Coordinates 681074..681388 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KFU91_RS03315 Protein ID WP_000560255.1
Coordinates 681385..681801 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU91_RS03290 (677232) 677232..678218 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
KFU91_RS03295 (678297) 678297..678989 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
KFU91_RS03300 (679066) 679066..679569 - 504 WP_001300832.1 M48 family metallopeptidase -
KFU91_RS03305 (679654) 679654..680790 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KFU91_RS03310 (681074) 681074..681388 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KFU91_RS03315 (681385) 681385..681801 + 417 WP_000560255.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KFU91_RS03320 (681846) 681846..683864 - 2019 WP_061355459.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KFU91_RS03325 (684290) 684290..686641 - 2352 WP_000695486.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T200594 WP_000550189.1 NZ_CP073929:681074-681388 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T200594 NZ_CP098030:c3921490-3921101 [Serratia ureilytica]
ATGTACATGTTTGATACCAACACCGTCAGCCACTTGTTTCGGCAGCATCCGCAGGTGCTTAACGTCATGGAAAAGCTCCC
GCCTTCTGCCGTATGTATTTCCAGCGTCACGGAGGCGGAACTGCTTTACGGTGTCGCTAAACGGCGGAACAAGGCGTTGC
AAAGCATGGTCGAGGCCTTTCTTGCCGCGGTGACAGTCTATGCATGGGATAGCGAAGCTGCCCGCTGTTACGGTGAAATG
CGGGCAAACATGGAGAGAAAGGGGAAAATCATGGGCGCGATGGATCAACTGATCGCCGCTCATGCGCAGAGCCGTGGCGC
GACGCTGGTGACCAACGATCGCGCATTCGCCATGGTGCCCGGATTGGCGGTTGAGGACTGGACGCACTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14951.41 Da        Isoelectric Point: 4.5577

>AT200594 WP_000560255.1 NZ_CP073929:681385-681801 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNAPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT200594 NZ_CP098030:c3921757-3921494 [Serratia ureilytica]
ATGGAAAGAATCGCAAAGCTGTTTAAAAACGGCAGAAATCAGGCGGTGAGGTTACCGGTGGAATTTGAATTTGATACGGA
TCGTGTCTATATCCGTCGCGATAGGGAGGGCAACGTGATTTTATCCAAATATCCAGCAAAGCCGGACAGCTGGGCACCGC
TGCTGAGCATTATCGCCCAAACGCCGGTGCCGCAAGAGTTTCTTGGCGCGGAAGATCGTCAGCAAGGGGTTGCAGACCGC
GATCCATTCTCAGAGGACGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References