Detailed information of TA system TA200587

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 73122..73376
• Replicon: plasmid pYLMB98a
• Accession: NZ_CP073927
• Organism: Escherichia coli strain MB98

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KFU77_RS23925
• Protein ID: WP_001312851.1
• Coordinates: 73122..73271 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	73315..73376 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU77_RS23895 (69145)	69145..69546	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
KFU77_RS23900 (69479)	69479..69736	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
KFU77_RS23905 (69829)	69829..70482	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
KFU77_RS23910 (71421)	71421..72278	-	858	WP_013362810.1	incFII family plasmid replication initiator RepA	-
KFU77_RS23915 (72271)	72271..72345	-	75	WP_032336874.1	RepA leader peptide Tap	-
KFU77_RS23920 (72581)	72581..72838	-	258	WP_000083833.1	replication regulatory protein RepA	-
KFU77_RS23925 (73122)	73122..73271	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (73315)	73315..73376	+	62	NuclAT_0	-	Antitoxin
- (73315)	73315..73376	+	62	NuclAT_0	-	Antitoxin
- (73315)	73315..73376	+	62	NuclAT_0	-	Antitoxin
- (73315)	73315..73376	+	62	NuclAT_0	-	Antitoxin
KFU77_RS23930 (73515)	73515..73697	-	183	WP_000968309.1	hypothetical protein	-
KFU77_RS23935 (73798)	73798..74414	+	617	Protein_89	IS1-like element IS1A family transposase	-
KFU77_RS23940 (74452)	74452..76023	-	1572	WP_023149734.1	IS66-like element ISCro1 family transposase	-
KFU77_RS23945 (76043)	76043..76390	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
KFU77_RS23950 (76390)	76390..77067	-	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
KFU77_RS23955 (77122)	77122..77211	+	90	Protein_93	IS1 family transposase	-
KFU77_RS23960 (77512)	77512..77724	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(B) / mph(A) / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / sitABCD	iutA / iucD / iucC / iucB / iucA	1..118380	118380
	-	inside	IScluster/Tn	tet(B) / mph(A) / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr	-	35636..77037	41401

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T200587 WP_001312851.1 NZ_CP073927:c73271-73122 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T200587 NZ_CP098030:c1112048-1111845 [Serratia ureilytica]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAAAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCCGCCGATCATCGTTTGGCGGAGCTGACCATGAACAAGC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGATAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT200587 NZ_CP073927:73315-73376 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin