Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1176909..1177636 Replicon chromosome
Accession NZ_CP073926
Organism Escherichia coli strain MB98

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag KFU77_RS05755 Protein ID WP_000547564.1
Coordinates 1176909..1177220 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KFU77_RS05760 Protein ID WP_000126294.1
Coordinates 1177217..1177636 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU77_RS05725 (1172051) 1172051..1173760 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
KFU77_RS05730 (1173770) 1173770..1174312 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
KFU77_RS05735 (1174312) 1174312..1175079 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
KFU77_RS05740 (1175076) 1175076..1175486 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
KFU77_RS05745 (1175479) 1175479..1175949 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
KFU77_RS05750 (1175974) 1175974..1176735 + 762 WP_001026446.1 hypothetical protein -
KFU77_RS05755 (1176909) 1176909..1177220 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
KFU77_RS05760 (1177217) 1177217..1177636 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
KFU77_RS05765 (1177750) 1177750..1179174 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
KFU77_RS05770 (1179183) 1179183..1180640 - 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
KFU77_RS05775 (1180900) 1180900..1181910 + 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
KFU77_RS05780 (1182059) 1182059..1182586 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T200571 WP_000547564.1 NZ_CP073926:1176909-1177220 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T200571 NZ_CP098025:564131-564247 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT200571 WP_000126294.1 NZ_CP073926:1177217-1177636 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT200571 NZ_CP098025:c564363-564158 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAATAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References