Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 660330..661129 Replicon chromosome
Accession NZ_CP073926
Organism Escherichia coli strain MB98

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag KFU77_RS03220 Protein ID WP_000347251.1
Coordinates 660330..660794 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KFU77_RS03225 Protein ID WP_001307405.1
Coordinates 660794..661129 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU77_RS03190 (655331) 655331..655765 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KFU77_RS03195 (655783) 655783..656661 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KFU77_RS03200 (656651) 656651..657430 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KFU77_RS03205 (657441) 657441..657914 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KFU77_RS03210 (657937) 657937..659217 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
KFU77_RS03215 (659466) 659466..660275 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KFU77_RS03220 (660330) 660330..660794 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KFU77_RS03225 (660794) 660794..661129 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KFU77_RS03230 (661278) 661278..662849 - 1572 WP_001273761.1 galactarate dehydratase -
KFU77_RS03235 (663224) 663224..664558 + 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
KFU77_RS03240 (664574) 664574..665344 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T200566 WP_000347251.1 NZ_CP073926:c660794-660330 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T200566 NZ_CP097983:c2426713-2426612 [Pantoea alhagi]
GGCAAGGCGACATCGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTTACAAGAGCCATTTCCCTGGACCGAATATAG
GAATCGTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT200566 WP_001307405.1 NZ_CP073926:c661129-660794 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT200566 NZ_CP097983:2426612-2426713 [Pantoea alhagi]
AAAAAAAGACCGAATACGATTCCTATATTCGGTCCAGGGAAATGGCTCTTGTAAGAGCCGTGCGCTAAAAGTTGGCATTA
ATGCAGGCGATGTCGCCTTGCC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References