Detailed information of TA system TA200563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4620348..4620950
• Replicon: chromosome
• Accession: NZ_CP073924
• Organism: Escherichia coli strain MB165

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KFU71_RS22125
• Protein ID: WP_000897305.1
• Coordinates: 4620639..4620950 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KFU71_RS22120
• Protein ID: WP_000356397.1
• Coordinates: 4620348..4620638 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU71_RS22095 (4616293)	4616293..4617195	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KFU71_RS22100 (4617192)	4617192..4617827	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KFU71_RS22105 (4617824)	4617824..4618753	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KFU71_RS22110 (4619083)	4619083..4619325	-	243	WP_001086388.1	protein YiiF	-
KFU71_RS22115 (4619544)	4619544..4619762	-	219	WP_001611167.1	CopG family transcriptional regulator	-
KFU71_RS22120 (4620348)	4620348..4620638	-	291	WP_000356397.1	NadS family protein	Antitoxin
KFU71_RS22125 (4620639)	4620639..4620950	-	312	WP_000897305.1	hypothetical protein	Toxin
KFU71_RS22130 (4621179)	4621179..4622087	+	909	WP_001162704.1	alpha/beta hydrolase	-
KFU71_RS22135 (4622151)	4622151..4623092	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KFU71_RS22140 (4623137)	4623137..4623574	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KFU71_RS22145 (4623571)	4623571..4624443	-	873	WP_000920762.1	virulence factor BrkB family protein	-
KFU71_RS22150 (4624437)	4624437..4625036	-	600	WP_001295269.1	glucose-1-phosphatase	-
KFU71_RS22155 (4625135)	4625135..4625920	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T200563 WP_000897305.1 NZ_CP073924:c4620950-4620639 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T200563 NZ_CP097983:1040272-1040790 [Pantoea alhagi]
TTGGACACTTTAAAAGTTGAGCTTTTCACGGATGAAGCTCAGTACAAAATAAATAACTTTGATTGTGGCAAAACAACTTT
AAATAAATTTCTGCTCGAGCATCTCAGACGTCAACATAACGGGAAAATATTAAGAGCGTATGTGCTGATAAATCACGAGG
CGGAGCCAAAAGTCCTGGGCTATTACACGTTATCCGGCGGTAGTTTTGAAAGATCTAGCTTGCCCTCTAAAACGCAACAG
AAAAAAATCCCATATAAAAATGTGCCATGCGTTACCCTTGGCAGGCTGGCGATAGATAAAACTCTGCATGGGCAAGGCTG
GGGAAGCATGTTGGTTTCTCATGCGATGAGCGTTGTTTATCACGCGTCATTGGCGGTAGGTATACATGGCATGTTTGTCG
ACGCCCTGGATGATGAGGCTAAACAATTTTATACCAGTCTCGGTTTTATTGAATTGGCAGGTGAAAACGGCAGTTCATTA
TTTTATCCAATACAATCCATTGAAAAATTATTTCAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT200563 WP_000356397.1 NZ_CP073924:c4620638-4620348 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT200563 NZ_CP097983:1039996-1040268 [Pantoea alhagi]
ATGGCAAACACATTAAAGAAACAACGCATCGATCTGCGTTTATCTCCAGAAGATAAAACGCTTATCGAAAACGCGGCCTC
TCTCAGCAATCTAACCATCTCGCAATTTATGCTGGCAAGTGCTTCTGAACGAGCAGCTGAAGTCATTGAACAACATCAAC
GTATAGTGCTCAATGAAGCATCATGGAAGCAGGTTATGGAGGCCATCAGCAATCCACCCGAACCTAATGTAAAGTTAAAG
CGAGCGGCCCAACGTCTACAGAACATGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7