200562

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4337111..4337608 Replicon chromosome
Accession NZ_CP073924
Organism Escherichia coli strain MB165

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KFU71_RS20870 Protein ID WP_001173343.1
Coordinates 4337111..4337284 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KFU71_RS20875 Protein ID WP_000398619.1
Coordinates 4337312..4337608 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU71_RS20860 (4333781) 4333781..4335238 + 1458 WP_257187638.1 dipeptide/tripeptide permease DtpC -
KFU71_RS20865 (4335475) 4335475..4336992 + 1518 WP_001295074.1 lysine--tRNA ligase -
KFU71_RS20870 (4337111) 4337111..4337284 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KFU71_RS20875 (4337312) 4337312..4337608 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KFU71_RS20880 (4337835) 4337835..4338107 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KFU71_RS20885 (4338119) 4338119..4338349 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
KFU71_RS20890 (4338530) 4338530..4340161 + 1632 WP_001216487.1 sensor histidine kinase -
KFU71_RS20895 (4340158) 4340158..4340877 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4328258..4337608 9350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T200562 WP_001173343.1 NZ_CP073924:c4337284-4337111 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T200562 NZ_CP097983:595951-596226 [Pantoea alhagi]
ATGGAAGTTTACTGGACGCTTAAAGCTCAGGATGACCTGGAGCGTATCTACCGTTTTGCCCTTCAGTACTGCCGCCAGCA
CGCCGATGACGTTTTGGATCGCCTGATTACTGGTTGTGCTGGTCTTACAGCGCATCCGGCAATTGGCGTACAGCAGACTC
GGTATGAACCTCGTGAGGTCCGAAAAGTATTATTCGATGATTACGAGGTTCACTACGAACTTCGCGATAATGACATCTAT
ATCGTGGATCTCTGGCATACCAAAGAAGAACGATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT200562 WP_000398619.1 NZ_CP073924:c4337608-4337312 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT200562 NZ_CP097983:595699-595947 [Pantoea alhagi]
ATGGGTTCGCTTACTAAACAAACCATCAGCGCACAGATACCCGTTGAGTTGGCTGCTGCTGTTGAAAATCTGGCAATTGA
GTTGGACCGTTCTAAGAGCTGGATTATTAAAGAAGCGTTGACCAGTATGCTGGCCGAAAGAGAACGGCGTCATCAGAGCA
TTCAGGCCGGTCTTGCCGATGTGGATGCTGGTCGCGTGGTGAGCCACTCGGACATGGTGGATTTTGCTAATCGCTTAAAG
AAATCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References