Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3730059..3730600 Replicon chromosome
Accession NZ_CP073924
Organism Escherichia coli strain MB165

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag KFU71_RS18005 Protein ID WP_000615982.1
Coordinates 3730322..3730600 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KFU71_RS18000 Protein ID WP_000729704.1
Coordinates 3730059..3730319 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU71_RS17975 (3725842) 3725842..3726627 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
KFU71_RS17980 (3726599) 3726599..3728311 + 1713 Protein_3519 flagellar biosynthesis protein FlhA -
KFU71_RS17985 (3728416) 3728416..3728694 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
KFU71_RS17990 (3728687) 3728687..3729043 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
KFU71_RS17995 (3729100) 3729100..3729873 - 774 WP_000543899.1 C40 family peptidase -
KFU71_RS18000 (3730059) 3730059..3730319 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KFU71_RS18005 (3730322) 3730322..3730600 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KFU71_RS18010 (3730756) 3730756..3731496 + 741 WP_257187627.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KFU71_RS18015 (3731467) 3731467..3732234 - 768 WP_000333380.1 class II glutamine amidotransferase -
KFU71_RS18020 (3732440) 3732440..3733018 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T200559 WP_000615982.1 NZ_CP073924:3730322-3730600 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T200559 NZ_CP097897:1883006-1883281 [Acetobacterium wieringae]
ATGAAATACGAAGTTAAATTCACAAATCAGTTTAAAAAAGATCTTAAGCTTGCCAAAAAACAAGGCAGAGATATTGACCG
ACTTTATGCTGTTATTGAAAAACTTGCCGATGATGAACCTCTGGAACAAAAATATCGTGATCATGATTTAAGTGGTAATT
ATAAAGGATGCCGTGAATGTCATGTCGAACCCGATTGGCTTTTGGTCTATGAGGTGATGGACGATGTTCTGGTATTAATG
CTTTATCGTATCGGAAGTCACTCAACGTTGTTCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT200559 WP_000729704.1 NZ_CP073924:3730059-3730319 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT200559 NZ_CP097897:1882734-1883009 [Acetobacterium wieringae]
ATGGCTACTAGTACAAATTTGAATATTCGTACCGATAAAGATATCAAAGAAGCTGCAGAGAAAATTTTTGGAGAGCTTGG
TCTTAACATGACAACAGCAATCAATATTTTTCTCAGACAAGCAATCCGGGAAAATGGGATACCTTTTGAAATGAAATTGT
CTGTTCCAAATCAGGAAACAGTTGCTGCCATTACCGAAGGTCGAAAACTGGCCAAAGATAGCAGTGTCAAAGGTTATTCC
AATATGGATGATTTGCGAGCTGCACTGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References