Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1889280..1889782 Replicon chromosome
Accession NZ_CP073924
Organism Escherichia coli strain MB165

Toxin (Protein)


Gene name yoeB Uniprot ID A0A8E0FJZ4
Locus tag KFU71_RS09045 Protein ID WP_000767833.1
Coordinates 1889528..1889782 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag KFU71_RS09040 Protein ID WP_001259255.1
Coordinates 1889280..1889531 (+) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU71_RS09015 (1884459) 1884459..1885526 - 1068 WP_072652219.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
KFU71_RS09020 (1885526) 1885526..1886596 - 1071 WP_000108969.1 histidinol-phosphate transaminase -
KFU71_RS09025 (1886593) 1886593..1887897 - 1305 WP_072652220.1 histidinol dehydrogenase -
KFU71_RS09030 (1887903) 1887903..1888802 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
KFU71_RS09035 (1888948) 1888948..1888998 - 51 WP_001364200.1 his operon leader peptide -
KFU71_RS09040 (1889280) 1889280..1889531 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
KFU71_RS09045 (1889528) 1889528..1889782 + 255 WP_000767833.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
KFU71_RS09050 (1889865) 1889865..1890689 + 825 WP_032202495.1 SDR family oxidoreductase -
KFU71_RS09055 (1890735) 1890735..1891664 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
KFU71_RS09060 (1891879) 1891879..1891941 + 63 WP_010723108.1 membrane protein YoeI -
KFU71_RS09065 (1891931) 1891931..1893289 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
KFU71_RS09070 (1893468) 1893468..1894526 + 1059 WP_000492305.1 thiosulfate utilization transporter TsuA/YeeE -
KFU71_RS09075 (1894540) 1894540..1894767 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-84)

Antitoxin

(1-73)


Sequences


Toxin        


Download         Length: 85 a.a.        Molecular weight: 10173.54 Da        Isoelectric Point: 8.0353

>T200546 WP_000767833.1 NZ_CP073924:1889528-1889782 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPESLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T200546 NZ_CP097896:992042-993376 [Pectobacterium actinidiae]
ATGCCTCGAATACAACAGCGTTTAGCAATATGGATGAATGGAATTCAAGTGGGATTCTGGGAAAAGAGCCGAGGCGAAGA
TAGGTTGCAATATCTTCCCGAATGGATCGCTGATGAACAAGGAAGACCTTTATCGCTTTCTTTGCTTTTCACCCCCGGTA
ATCAGATTTGGCGGGGCAATGTGGTACGCGACTATTTTGATAATTTATTGCCTGACAGCGAAGGTATACGCAGGCGTTTA
GCGACGCGTTACCACGCTGATAGCCTTGAGCCTTTTGATCTGTTGGCTGAGCTGGGGAGAGACTGTGTTGGTGCGATACA
GTTGCTGAATGGTAATGAGGAGCCTACAGATCTCTTTTCCGTAAAGTATCGCCCGATTTGTGACGCAGAGATTGCCGCTA
TATTGCGTAATACAACAGCGGCATTGTTGCCAGGTCGGCAGGATGATACTGACGATTTACGTTTATCGATTGCCGGTGCG
CAGGAAAAAACGGCTTTACTCTGGCATGAAAGGCAATGGTGTTTGCCAGAAGGGAGTACCCCTACGACGCATATTTTCAA
GTTACCACTCGGGCTAGTGGGTAATATGCAAGCGGATATGAGTACGTCGGTTGAAAATGAATGGCTGTGTTCTTTGATCC
TTAAGGAATATGGAATTCCTGTTGCAAAAACACAGATTGCGCAGTTTGAAGATCAGAAAGTATTGGTTGTTGAGCGTTTT
GACAGAAGATGGTCAAGCGATCAGCAATGGATCATTCGCTTGCCACAAGAGGATATGTGTCAGGCTCTGGGTGTTTCCCC
GCTACGAAAATACCAGTCTGATGGTGGGCCAGGGATTTCCGATATTATGACCATACTGAGTCATTCAGAGCAGGCAGAGC
AGGACAAAGCGCAGTTTTTCAGGGCGCAAATTATTTTCTGGCTGATAGCGGCTACTGATGGGCACGCCAAAAATTTCAGT
ATCGCTATTGAGCCACAAGGTCGCTATCACCTTACGCCTCTTTATGATGTTTTATCGGCATGGCCGGTCATTGGTCCGCG
TAATAACCAGATTTCCTGGCAGAAGTGCAAGCTGGCAATGGCTGTTCGGGGTAGTAGTAATTATTACCATCTGTACAGAA
TTCAACGGCGGCATTGGGTCAATCATGGTGAATTAACTGGTCTGGGTAGACGACAGGTTGAAGCCATGATGGATGATATT
ATCTTCAGGACACCTGAGGTTATTGAACGTGTATCTTCGTTGCTGCCTGAGTCATTCTCACCAGAGCTTGCGGAATGTGT
TTTTGACGGTATGCGACAACAGTGTGGTCGGTTACAAGAATTGCACCGTAAATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT200546 WP_001259255.1 NZ_CP073924:1889280-1889531 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT200546 NZ_CP097896:991732-992037 [Pectobacterium actinidiae]
ATGAATACAGTCTATCCGTTGAAAACCTTGAATCAGTTGCGCCCATTGCTGATTGGCTTTCGCAAGGCTAAAGGACTGAC
GCAAAAAGACGTGTCTGAAAGGTTAGGTGTAACACAACAAACCTATGCCCGTTTGGAAGCAAACCCCGGAAGTGCCAGTA
TTGAGCGCTTGTTTAAAGTGCTCACCGTTCTGGGGGTTGAGGTAGTGCTGTCTTCTGGACCAAGAGTACATTTCTCGATG
TCGTCCTCAACAATGGAAGAGCCAGAAAAGCCAATAGACTCACCCGCCAGACGGGAAAAATGGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References