Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 617031..617830 Replicon chromosome
Accession NZ_CP073924
Organism Escherichia coli strain MB165

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag KFU71_RS03000 Protein ID WP_000347266.1
Coordinates 617031..617495 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KFU71_RS03005 Protein ID WP_001307405.1
Coordinates 617495..617830 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU71_RS02970 (612032) 612032..612466 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KFU71_RS02975 (612484) 612484..613362 - 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
KFU71_RS02980 (613352) 613352..614131 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KFU71_RS02985 (614142) 614142..614615 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KFU71_RS02990 (614638) 614638..615918 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
KFU71_RS02995 (616167) 616167..616976 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KFU71_RS03000 (617031) 617031..617495 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KFU71_RS03005 (617495) 617495..617830 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KFU71_RS03010 (617979) 617979..619550 - 1572 WP_001273741.1 galactarate dehydratase -
KFU71_RS03015 (619925) 619925..621259 + 1335 WP_000599640.1 galactarate/glucarate/glycerate transporter GarP -
KFU71_RS03020 (621275) 621275..622045 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T200541 WP_000347266.1 NZ_CP073924:c617495-617031 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T200541 NZ_CP097895:508911-509261 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT200541 WP_001307405.1 NZ_CP073924:c617830-617495 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT200541 NZ_CP097895:508624-508905 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References