Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 419715..420279 Replicon chromosome
Accession NZ_CP073916
Organism Lacticaseibacillus paracasei subsp. tolerans strain 2A

Toxin (Protein)


Gene name yafQ Uniprot ID A0A6G9EML4
Locus tag KFU60_RS02235 Protein ID WP_014951859.1
Coordinates 419977..420279 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID K6SLT0
Locus tag KFU60_RS02230 Protein ID WP_003570051.1
Coordinates 419715..419984 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU60_RS02200 (KFU60_02200) 415056..415943 - 888 WP_016385537.1 ABC transporter ATP-binding protein -
KFU60_RS02205 (KFU60_02205) 416140..417366 - 1227 WP_011674431.1 hypothetical protein -
KFU60_RS02210 (KFU60_02210) 417564..417968 + 405 WP_003594355.1 MerR family transcriptional regulator -
KFU60_RS02215 (KFU60_02215) 417961..418287 + 327 WP_211772764.1 YrdB family protein -
KFU60_RS02220 (KFU60_02220) 418349..418643 + 295 Protein_441 hypothetical protein -
KFU60_RS02225 (KFU60_02225) 418688..419341 + 654 WP_003570049.1 N-acetyltransferase -
KFU60_RS02230 (KFU60_02230) 419715..419984 + 270 WP_003570051.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
KFU60_RS02235 (KFU60_02235) 419977..420279 + 303 WP_014951859.1 type II toxin-antitoxin system YafQ family toxin Toxin
KFU60_RS02240 (KFU60_02240) 420618..420761 + 144 WP_003574774.1 hypothetical protein -
KFU60_RS02245 (KFU60_02245) 420988..421575 + 588 WP_003574776.1 hypothetical protein -
KFU60_RS02250 (KFU60_02250) 421811..423988 + 2178 WP_211772765.1 Tex family protein -
KFU60_RS02255 (KFU60_02255) 424163..424600 - 438 WP_016385305.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-98)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(10-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11441.27 Da        Isoelectric Point: 10.2818

>T200538 WP_014951859.1 NZ_CP073916:419977-420279 [Lacticaseibacillus paracasei subsp. tolerans]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELHVDGPRGDWLLIYKIK
QQDLILTLVRTGSHHNLLGK

Download         Length: 303 bp

>T200538 NZ_CP097885:1392053-1392238 [Peptoniphilus sp. SAHP1]
ATGCCGATGACACAAGATGAAATGGTCAAGCTACTAAGAAAGAATGGTTTCGAAGTAGTTAAGGGTGGCAAGGGCAGTCA
TATAAAGATGACAAAACTTGGCATCAAAAGACCAATCATTATCCCACATGGAGAACTCCAAAAGGGAACCGAGCGTGGGA
TACTAAAAGAGGCAGGGCTAAAATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9982.68 Da        Isoelectric Point: 7.2026

>AT200538 WP_003570051.1 NZ_CP073916:419715-419984 [Lacticaseibacillus paracasei subsp. tolerans]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Download         Length: 270 bp

>AT200538 NZ_CP097885:1392271-1392711 [Peptoniphilus sp. SAHP1]
ATGTTAGTTTCTTATCCAGCTATATTTTATTATAGTCCAGAAGAGAAGGGTTACTATGTTTATATGCCAGACATTGGAGG
TGCAGGGACTCAAGGCAATTCAATAGAGGAAGCTTTGTTAATGGCAAGTGACTACTTGGGAATAATGGCTAGCTCTATAA
TAGAAGGCGGAGATACTTTGCCAAAACAATCAGCCATAAGTGACTTGTCTATTGAAGATGATTTTCCTTTTAAAGATGAT
GATGATTTTGATGGCTTCTATGATTATTCTAAGTCCTTTGTTTCTCTCGTTTATGTTGATTTAAAGGACTATTTAGGTAG
CCAAGAACTTGTAAAAAAGACCTTATCTATACCCAAGTGGAGCAATGATCTAGGGAATAAATTGAATTTGAATTTTTCTA
AGTTACTGACTGAAGCTATTGTAAATATCGCCAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6G9EML4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BRK6

References