Detailed information of TA system TA200536

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-DinJ
• Location: 173594..174079
• Replicon: chromosome
• Accession: NZ_CP073915
• Organism: Lacticaseibacillus rhamnosus strain AS

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: KFU59_RS13770
• Protein ID: WP_249146496.1
• Coordinates: 173594..173701 (-)
• Length: 36 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A0A807RQZ2
• Locus tag: KFU59_RS00780
• Protein ID: WP_005684270.1
• Coordinates: 173807..174079 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU59_RS00760 (KFU59_00760)	168911..169747	-	837	WP_014569137.1	Rgg/GadR/MutR family transcriptional regulator	-
KFU59_RS00765 (KFU59_00765)	169795..171210	-	1416	WP_015764852.1	IS5-like element ISLrh2 family transposase	-
KFU59_RS00770 (KFU59_00770)	171961..173370	-	1410	WP_211751532.1	3'-5' exonuclease	-
KFU59_RS13770	173594..173701	-	108	WP_249146496.1	hypothetical protein	Toxin
KFU59_RS00780 (KFU59_00780)	173807..174079	-	273	WP_005684270.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
KFU59_RS00785 (KFU59_00785)	174192..174485	+	294	WP_005684269.1	metalloregulator ArsR/SmtB family transcription factor	-
KFU59_RS00790 (KFU59_00790)	174567..175730	+	1164	WP_005691640.1	MFS transporter	-
KFU59_RS00795 (KFU59_00795)	175884..176459	+	576	WP_211751533.1	DNA-3-methyladenine glycosylase I	-
KFU59_RS00800 (KFU59_00800)	176581..176799	+	219	WP_005691646.1	hypothetical protein	-
KFU59_RS00805 (KFU59_00805)	176928..177809	-	882	WP_005684263.1	class II fructose-1,6-bisphosphate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (5-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-84)

Sequences

Toxin

Protein

• Length: 36 a.a.
• Molecular weight: 3982.71 Da
• Isoelectric Point: 4.6286

>T200536 WP_249146496.1 NZ_CP073915:c173701-173594 [Lacticaseibacillus rhamnosus]
MRVAPDWLLVYKVDAEALILMLLATGTHRDTLNIE

Nucleotide

• Length: 108 bp

>T200536 NZ_CP097884:4296568-4296846 [Escherichia coli str. K-12 substr. MG1655]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10323.01 Da
• Isoelectric Point: 10.7322

>AT200536 WP_005684270.1 NZ_CP073915:c174079-173807 [Lacticaseibacillus rhamnosus]
MAKESRIRNWINTITRKRALHVLNRLGLERFSAINMYWKRIGDTGALPFTLEMSFADQLRFAEADVKAGRIKSFKTVGAL
MKDLYSDVDG

Nucleotide

• Length: 273 bp

>AT200536 NZ_CP097884:4296305-4296565 [Escherichia coli str. K-12 substr. MG1655]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A807RQZ2