200528

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/VapI(antitoxin)
Location 4269610..4271057 Replicon chromosome
Accession NZ_CP073910
Organism Sphingobium phenoxybenzoativorans strain Tas13

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag KFK14_RS21215 Protein ID WP_212608999.1
Coordinates 4269610..4269945 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KFK14_RS21220 Protein ID WP_212609000.1
Coordinates 4269960..4271057 (+) Length 366 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFK14_RS21180 (KFK14_21180) 4265041..4266384 - 1344 WP_212608996.1 TraB/VirB10 family protein -
KFK14_RS21185 (KFK14_21185) 4266384..4267190 - 807 WP_212608997.1 type-F conjugative transfer system secretin TraK -
KFK14_RS21190 (KFK14_21190) 4267199..4267762 - 564 WP_030538243.1 type IV conjugative transfer system protein TraE -
KFK14_RS21195 (KFK14_21195) 4267775..4268062 - 288 WP_070157786.1 type IV conjugative transfer system protein TraL -
KFK14_RS21200 (KFK14_21200) 4268097..4268411 - 315 WP_070157785.1 hypothetical protein -
KFK14_RS21205 (KFK14_21205) 4268589..4269272 + 684 WP_246524724.1 hypothetical protein -
KFK14_RS21210 (KFK14_21210) 4269265..4269480 + 216 WP_212608998.1 AlpA family transcriptional regulator -
KFK14_RS21215 (KFK14_21215) 4269610..4269945 + 336 WP_212608999.1 hypothetical protein Toxin
KFK14_RS21220 (KFK14_21220) 4269960..4271057 + 1098 WP_212609000.1 HigA family addiction module antitoxin Antitoxin
KFK14_RS21225 (KFK14_21225) 4271109..4271732 - 624 WP_212609001.1 S24 family peptidase -
KFK14_RS21230 (KFK14_21230) 4271905..4272954 + 1050 WP_212609002.1 IS110 family transposase -
KFK14_RS21235 (KFK14_21235) 4273099..4273857 - 759 WP_212609004.1 arsenical resistance protein ArsH -
KFK14_RS21240 (KFK14_21240) 4273860..4274927 - 1068 WP_212609005.1 ACR3 family arsenite efflux transporter -
KFK14_RS21245 (KFK14_21245) 4274943..4275374 - 432 WP_212609006.1 arsenate reductase (glutaredoxin) -
KFK14_RS21250 (KFK14_21250) 4275371..4275892 - 522 WP_212609007.1 arsenate reductase ArsC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 4107352..4300100 192748


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

IrrE N-terminal-like domain

(226-315)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12821.87 Da        Isoelectric Point: 8.5307

>T200528 WP_212608999.1 NZ_CP073910:4269610-4269945 [Sphingobium phenoxybenzoativorans]
MDIDFRKRKLEKTFNAQAELLKAYGLPMAKAIMKRMTVLRAARTLSQVPITPPERRHQLTEDRDEQYAVDLVHPKRLVFE
VNHDPIPRSEDGGIDLTKVTAITIIEVVDYH

Download         Length: 336 bp

>T200528 NZ_CP097884:2847815-2848102 [Escherichia coli str. K-12 substr. MG1655]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 366 a.a.        Molecular weight: 40409.04 Da        Isoelectric Point: 7.4847

>AT200528 WP_212609000.1 NZ_CP073910:4269960-4271057 [Sphingobium phenoxybenzoativorans]
MATAANQFRPDYAVPPGWILEERLEVHGMSQAEFARRCGRSAKLISEIIAGKAPLEPETAIQFEKVLGVDADLWLSIESG
YQLHRAREADAARAKDAIAWSKTFPINDLVKRRCFAKPVNPVDAVSKLLAFFGVGSLDAWNSQYGLAKVAYRHSPSFKSN
EVALATWLRLGELDAEQQECADYSETQFKLALKQIRALTREPVEQAIPRTRALCNEAGVALALVPPLAKTALSGAAWWIS
PRKPVIQLSARHKSDDHLWFSFFHEAAHILLHSKKSVFVDDKSRDGTDEEKEADAWACNTLVSTSAWRQFVGTGPRSERA
VLDFAESQGIAPGIIVGMLQHSGILPWTHLNGLKTRLKWSTGAED

Download         Length: 1098 bp

>AT200528 NZ_CP097884:2847576-2847815 [Escherichia coli str. K-12 substr. MG1655]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References