Detailed information of TA system TA200520

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2257..2521
• Replicon: plasmid pHK31_3
• Accession: NZ_CP073909
• Organism: Klebsiella pneumoniae strain HK31

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: -
• Locus tag: KFK82_RS28135
• Protein ID: WP_024179505.1
• Coordinates: 2369..2521 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	2257..2317 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFK82_RS28130 (869)	869..2077	-	1209	WP_064148469.1	IncI1-type conjugal transfer protein TrbA	-
- (2257)	2257..2317	-	61	NuclAT_0	-	Antitoxin
- (2257)	2257..2317	-	61	NuclAT_0	-	Antitoxin
- (2257)	2257..2317	-	61	NuclAT_0	-	Antitoxin
- (2257)	2257..2317	-	61	NuclAT_0	-	Antitoxin
KFK82_RS28135 (2369)	2369..2521	+	153	WP_024179505.1	Hok/Gef family protein	Toxin
KFK82_RS28140 (2593)	2593..2844	-	252	WP_001291962.1	hypothetical protein	-
- (3237)	3237..3288	-	52	NuclAT_1	-	-
- (3237)	3237..3288	-	52	NuclAT_1	-	-
- (3237)	3237..3288	-	52	NuclAT_1	-	-
- (3237)	3237..3288	-	52	NuclAT_1	-	-
KFK82_RS28145 (4180)	4180..4356	-	177	WP_180335155.1	hypothetical protein	-
KFK82_RS28150 (4749)	4749..4958	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
KFK82_RS28155 (5026)	5026..5693	-	668	Protein_6	plasmid IncI1-type surface exclusion protein ExcA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / blaCTX-M-104	-	1..98579	98579

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5743.07 Da
• Isoelectric Point: 8.7948

>T200520 WP_024179505.1 NZ_CP073909:2369-2521 [Klebsiella pneumoniae]
MPQRTFLMMLIVVCVTILCFVWIVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T200520 NZ_CP097884:1468041-1468448 [Escherichia coli str. K-12 substr. MG1655]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT200520 NZ_CP073909:c2317-2257 [Klebsiella pneumoniae]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Antitoxin