Detailed information of TA system TA020052

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/-
• Location: 245960..246501
• Replicon: chromosome
• Accession: HG738867
• Organism: Escherichia coli str. K-12 substr. MC4100

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: BN896_0211
• Protein ID: CDJ70799.1
• Coordinates: 245960..246238 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: BN896_0212
• Protein ID: CDJ70800.1
• Coordinates: 246241..246501 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN896_0208	243542..244120	+	579	CDJ70796.1	phosphoheptose isomerase	-
BN896_0209	244326..245093	+	768	CDJ70797.1	putative amidotransfease	-
BN896_0210	245064..245804	-	741	CDJ70798.1	hypothetical protein	-
BN896_0211	245960..246238	-	279	CDJ70799.1	putative toxin of the YafQ-DinJ toxin-antitoxin system	Toxin
BN896_0212	246241..246501	-	261	CDJ70800.1	putative antitoxin of YafQ-DinJ toxin-antitoxin system	Antitoxin
BN896_0213	246711..247460	+	750	CDJ70801.1	putative lipoprotein and C40 family peptidase	-
BN896_0214	247636..248133	+	498	CDJ70802.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (4-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10846.62 Da
• Isoelectric Point: 9.9941

>T20052 CDJ70799.1 HG738867:c246238-245960 [Escherichia coli str. K-12 substr. MC4100]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T20052 HG738867:c246238-245960 [Escherichia coli str. K-12 substr. MC4100]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9405.80 Da
• Isoelectric Point: 5.0758

>AT20052 CDJ70800.1 HG738867:c246501-246241 [Escherichia coli str. K-12 substr. MC4100]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT20052 HG738867:c246501-246241 [Escherichia coli str. K-12 substr. MC4100]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA