Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-doc
Location 821114..821739 Replicon chromosome
Accession NZ_CP073887
Organism Staphylococcus epidermidis strain V1933625

Toxin (Protein)


Gene name doc Uniprot ID Q5HMS9
Locus tag KFV30_RS04245 Protein ID WP_002504557.1
Coordinates 821344..821739 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A4Y7VKS9
Locus tag KFV30_RS04240 Protein ID WP_002504558.1
Coordinates 821114..821344 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFV30_RS04175 (KFV30_04175) 816183..816665 + 483 WP_002504570.1 hypothetical protein -
KFV30_RS04180 (KFV30_04180) 816681..816860 + 180 WP_002504569.1 hypothetical protein -
KFV30_RS04185 (KFV30_04185) 816845..817030 + 186 WP_001187008.1 hypothetical protein -
KFV30_RS04190 (KFV30_04190) 817046..817330 + 285 WP_002504568.1 hypothetical protein -
KFV30_RS04195 (KFV30_04195) 817327..817482 + 156 WP_002504567.1 transcriptional activator RinB -
KFV30_RS04200 (KFV30_04200) 817508..817780 + 273 WP_002504566.1 hypothetical protein -
KFV30_RS04205 (KFV30_04205) 817796..818338 + 543 WP_002504565.1 hypothetical protein -
KFV30_RS04210 (KFV30_04210) 818331..818507 + 177 WP_002504564.1 hypothetical protein -
KFV30_RS04215 (KFV30_04215) 818527..818748 + 222 WP_002504563.1 hypothetical protein -
KFV30_RS04220 (KFV30_04220) 818748..819473 + 726 WP_010959229.1 metallophosphoesterase -
KFV30_RS04225 (KFV30_04225) 819466..819783 + 318 WP_002504561.1 hypothetical protein -
KFV30_RS04230 (KFV30_04230) 819783..820433 + 651 WP_002504560.1 hypothetical protein -
KFV30_RS04235 (KFV30_04235) 820433..820951 + 519 WP_002504559.1 metallophosphoesterase -
KFV30_RS04240 (KFV30_04240) 821114..821344 + 231 WP_002504558.1 addiction module antitoxin Antitoxin
KFV30_RS04245 (KFV30_04245) 821344..821739 + 396 WP_002504557.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KFV30_RS04250 (KFV30_04250) 822042..822233 + 192 WP_002504556.1 hypothetical protein -
KFV30_RS04255 (KFV30_04255) 822217..822486 + 270 WP_000755772.1 hypothetical protein -
KFV30_RS04260 (KFV30_04260) 822543..822995 + 453 WP_257474725.1 hypothetical protein -
KFV30_RS04265 (KFV30_04265) 823040..823840 + 801 WP_000686449.1 metallophosphoesterase -
KFV30_RS04270 (KFV30_04270) 824035..824670 + 636 WP_227489978.1 hypothetical protein -
KFV30_RS04275 (KFV30_04275) 824776..825417 + 642 WP_002504537.1 hypothetical protein -
KFV30_RS13370 825448..825582 - 135 WP_002504536.1 hypothetical protein -
KFV30_RS04280 (KFV30_04280) 825665..826069 - 405 WP_002504535.1 YolD-like family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage aac(6')-aph(2'') - 720367..856099 135732


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-129)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14992.31 Da        Isoelectric Point: 9.4885

>T200464 WP_002504557.1 NZ_CP073887:821344-821739 [Staphylococcus epidermidis]
MQSTKYLTEKQVIAINVKAIQDFSPKEQVGVKVPEVLNATIEGVKQSFGGVELYETIERKAAFIYRNIAQKHAFHNANKR
TAFTSMVIFLKLNSINFECTQDEAVQFTLRVVNDKNLTLEGIETWIKRHCK

Download         Length: 396 bp

>T200464 NZ_CP097882:649276-649590 [Escherichia coli str. K-12 substr. MG1655]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8648.89 Da        Isoelectric Point: 5.1231

>AT200464 WP_002504558.1 NZ_CP073887:821114-821344 [Staphylococcus epidermidis]
MITTRKLRKAGNSSVVSVPTEVIAALGISNGDNLKFNVKDNKVTIEKEVREDEEFFKLLDETFTEYNQALKRMVDL

Download         Length: 231 bp

>AT200464 NZ_CP097882:649587-650003 [Escherichia coli str. K-12 substr. MG1655]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Y7VKP2

Antitoxin

Source ID Structure
AlphaFold DB A0A4Y7VKS9

References