Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/-
Location 3217820..3218547 Replicon chromosome
Accession CP009789
Organism Escherichia coli K-12 strain ER3413

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag ER3413_3175 Protein ID AIZ52877.1
Coordinates 3218233..3218547 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ER3413_3174 Protein ID AIZ52876.1
Coordinates 3217820..3218236 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ER3413_3171 3212980..3215331 + 2352 AIZ52874.1 alpha-glucosidase -
ER3413_3173 3215757..3217775 + 2019 AIZ52875.1 2,4-dienoyl-CoA reductase, NADH and FMN-linked -
ER3413_3174 3217820..3218236 - 417 AIZ52876.1 antitoxinof the HigB-HigA toxin-antitoxin system Antitoxin
ER3413_3175 3218233..3218547 - 315 AIZ52877.1 mRNA interferase toxin of the HigB-HigA toxin-antitoxin system Toxin
ER3413_3176 3218831..3219967 - 1137 AIZ52878.1 23S rRNA m(2)G1835 methyltransferase, SAM-dependent -
ER3413_3177 3220052..3220555 + 504 AIZ52879.1 putative metal dependent hydrolase -
ER3413_3178 3220632..3221324 + 693 AIZ52880.1 conserved protein, SanA family, DUF218 superfamily -
ER3413_3179 3221403..3222389 + 987 AIZ52881.1 putative NAD(P)-binding dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T20041 AIZ52877.1 CP009789:c3218547-3218233 [Escherichia coli K-12]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T20041 CP009789:c3218547-3218233 [Escherichia coli K-12]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT20041 AIZ52876.1 CP009789:c3218236-3217820 [Escherichia coli K-12]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT20041 CP009789:c3218236-3217820 [Escherichia coli K-12]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References