Detailed information of TA system TA020041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 3217820..3218547
• Replicon: chromosome
• Accession: CP009789
• Organism: Escherichia coli K-12 strain ER3413

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ER3413_3175
• Protein ID: AIZ52877.1
• Coordinates: 3218233..3218547 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ER3413_3174
• Protein ID: AIZ52876.1
• Coordinates: 3217820..3218236 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ER3413_3171	3212980..3215331	+	2352	AIZ52874.1	alpha-glucosidase	-
ER3413_3173	3215757..3217775	+	2019	AIZ52875.1	2,4-dienoyl-CoA reductase, NADH and FMN-linked	-
ER3413_3174	3217820..3218236	-	417	AIZ52876.1	antitoxinof the HigB-HigA toxin-antitoxin system	Antitoxin
ER3413_3175	3218233..3218547	-	315	AIZ52877.1	mRNA interferase toxin of the HigB-HigA toxin-antitoxin system	Toxin
ER3413_3176	3218831..3219967	-	1137	AIZ52878.1	23S rRNA m(2)G1835 methyltransferase, SAM-dependent	-
ER3413_3177	3220052..3220555	+	504	AIZ52879.1	putative metal dependent hydrolase	-
ER3413_3178	3220632..3221324	+	693	AIZ52880.1	conserved protein, SanA family, DUF218 superfamily	-
ER3413_3179	3221403..3222389	+	987	AIZ52881.1	putative NAD(P)-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12103.10 Da
• Isoelectric Point: 10.0409

>T20041 AIZ52877.1 CP009789:c3218547-3218233 [Escherichia coli K-12]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T20041 CP009789:c3218547-3218233 [Escherichia coli K-12]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14995.42 Da
• Isoelectric Point: 4.4547

>AT20041 AIZ52876.1 CP009789:c3218236-3217820 [Escherichia coli K-12]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT20041 CP009789:c3218236-3217820 [Escherichia coli K-12]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA