Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tsbAT/-
Location 1024298..1025100 Replicon chromosome
Accession NZ_CP073850
Organism Staphylococcus epidermidis strain B1266911

Toxin (Protein)


Gene name tsbT Uniprot ID Q5HN83
Locus tag KFV33_RS05520 Protein ID WP_002468490.1
Coordinates 1024921..1025100 (+) Length 60 a.a.

Antitoxin (Protein)


Gene name tsbA Uniprot ID Q5HN82
Locus tag KFV33_RS05515 Protein ID WP_002456349.1
Coordinates 1024298..1024897 (+) Length 200 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFV33_RS05490 (KFV33_05490) 1019509..1020966 + 1458 WP_002440300.1 ABC transporter substrate-binding protein/permease -
KFV33_RS05495 (KFV33_05495) 1020959..1021681 + 723 WP_001829838.1 amino acid ABC transporter ATP-binding protein -
KFV33_RS05500 (KFV33_05500) 1022178..1022333 + 156 WP_001829854.1 hypothetical protein -
KFV33_RS05505 (KFV33_05505) 1022543..1023673 + 1131 WP_001829814.1 tRNA epoxyqueuosine(34) reductase QueG -
KFV33_RS05510 (KFV33_05510) 1023670..1024140 + 471 WP_001829836.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
KFV33_RS05515 (KFV33_05515) 1024298..1024897 + 600 WP_002456349.1 glucosamine-6-phosphate isomerase Antitoxin
KFV33_RS05520 (KFV33_05520) 1024921..1025100 + 180 WP_002468490.1 SAS053 family protein Toxin
KFV33_RS05525 (KFV33_05525) 1025256..1025660 + 405 WP_001829818.1 hypothetical protein -
KFV33_RS05530 (KFV33_05530) 1025845..1027230 + 1386 WP_001829862.1 class II fumarate hydratase -
KFV33_RS05535 (KFV33_05535) 1027591..1028414 - 824 Protein_1032 RluA family pseudouridine synthase -
KFV33_RS05540 (KFV33_05540) 1028573..1029706 + 1134 WP_001829819.1 GAF domain-containing sensor histidine kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1022178..1022333 155


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Glucosamine-6-phosphate isomerase

(130-195)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6963.56 Da        Isoelectric Point: 4.3016

>T200395 WP_002468490.1 NZ_CP073850:1024921-1025100 [Staphylococcus epidermidis]
MANKKDSKLNYHEEENAMVTDLDDLKELGKEMEQISQENDEEKLNQSHDNEVRSDLKKQ

Download         Length: 180 bp

>T200395 NZ_CP097840:2904431-2904534 [Shigella flexneri]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 200 a.a.        Molecular weight: 22709.62 Da        Isoelectric Point: 4.9942

>AT200395 WP_002456349.1 NZ_CP073850:1024298-1024897 [Staphylococcus epidermidis]
MAMNFKVFDNSEKVAEYTADILRKQFNNNPTTIAGFHLSKEHAPVFDELKKNVENHTVDFSQINILDYDDNHSYYEALGV
PTGQIYSISYENDAIDFISDKIKTKENKGKLTMQVLTIDENGKLDISVRQGLMEAREIFLVITGANKRDMVEKLYRENGK
TSFEPSDLKAHRMVNVILDKEAAAGLPEDVKEYFTARFA

Download         Length: 600 bp

>AT200395 NZ_CP097840:c2904535-2904301 [Shigella flexneri]
TAAAAAAAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCA
TTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAAT
AAAAAGCGCGGTGCTCATCAGCTTAAATGTTAAAAACCGCCCGTTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HY44

Antitoxin

Source ID Structure
AlphaFold DB A0A5E9LUD0

References