Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tsbAT/-
Location 1021754..1022556 Replicon chromosome
Accession NZ_CP073824
Organism Staphylococcus epidermidis strain B1200599

Toxin (Protein)


Gene name tsbT Uniprot ID Q5HN83
Locus tag KFV28_RS05445 Protein ID WP_002468490.1
Coordinates 1022377..1022556 (+) Length 60 a.a.

Antitoxin (Protein)


Gene name tsbA Uniprot ID Q5HN82
Locus tag KFV28_RS05440 Protein ID WP_002456349.1
Coordinates 1021754..1022353 (+) Length 200 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFV28_RS05415 (KFV28_05415) 1016965..1018422 + 1458 WP_002440300.1 ABC transporter substrate-binding protein/permease -
KFV28_RS05420 (KFV28_05420) 1018415..1019137 + 723 WP_001829838.1 amino acid ABC transporter ATP-binding protein -
KFV28_RS05425 (KFV28_05425) 1019652..1019789 + 138 WP_064783702.1 hypothetical protein -
KFV28_RS05430 (KFV28_05430) 1019999..1021129 + 1131 WP_001829814.1 tRNA epoxyqueuosine(34) reductase QueG -
KFV28_RS05435 (KFV28_05435) 1021126..1021596 + 471 WP_001829836.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
KFV28_RS05440 (KFV28_05440) 1021754..1022353 + 600 WP_002456349.1 glucosamine-6-phosphate isomerase Antitoxin
KFV28_RS05445 (KFV28_05445) 1022377..1022556 + 180 WP_002468490.1 SAS053 family protein Toxin
KFV28_RS05450 (KFV28_05450) 1022712..1023116 + 405 WP_001829818.1 hypothetical protein -
KFV28_RS05455 (KFV28_05455) 1023301..1024686 + 1386 WP_001829862.1 class II fumarate hydratase -
KFV28_RS05460 (KFV28_05460) 1025047..1025870 - 824 Protein_1032 RluA family pseudouridine synthase -
KFV28_RS05465 (KFV28_05465) 1026029..1027162 + 1134 WP_001829819.1 GAF domain-containing sensor histidine kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1019634..1019789 155


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Glucosamine-6-phosphate isomerase

(130-195)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6963.56 Da        Isoelectric Point: 4.3016

>T200350 WP_002468490.1 NZ_CP073824:1022377-1022556 [Staphylococcus epidermidis]
MANKKDSKLNYHEEENAMVTDLDDLKELGKEMEQISQENDEEKLNQSHDNEVRSDLKKQ

Download         Length: 180 bp

>T200350 NZ_CP097834:c1169534-1169247 [Shigella flexneri]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 200 a.a.        Molecular weight: 22709.62 Da        Isoelectric Point: 4.9942

>AT200350 WP_002456349.1 NZ_CP073824:1021754-1022353 [Staphylococcus epidermidis]
MAMNFKVFDNSEKVAEYTADILRKQFNNNPTTIAGFHLSKEHAPVFDELKKNVENHTVDFSQINILDYDDNHSYYEALGV
PTGQIYSISYENDAIDFISDKIKTKENKGKLTMQVLTIDENGKLDISVRQGLMEAREIFLVITGANKRDMVEKLYRENGK
TSFEPSDLKAHRMVNVILDKEAAAGLPEDVKEYFTARFA

Download         Length: 600 bp

>AT200350 NZ_CP097834:c1169773-1169534 [Shigella flexneri]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HY44

Antitoxin

Source ID Structure
AlphaFold DB A0A5E9LUD0

References