Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tsbAT/-
Location 1024311..1025113 Replicon chromosome
Accession NZ_CP073821
Organism Staphylococcus epidermidis strain B1200343

Toxin (Protein)


Gene name tsbT Uniprot ID Q5HN83
Locus tag KFV43_RS05455 Protein ID WP_002468490.1
Coordinates 1024934..1025113 (+) Length 60 a.a.

Antitoxin (Protein)


Gene name tsbA Uniprot ID Q5HN82
Locus tag KFV43_RS05450 Protein ID WP_002456349.1
Coordinates 1024311..1024910 (+) Length 200 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFV43_RS05425 (KFV43_05425) 1019522..1020979 + 1458 WP_002440300.1 ABC transporter substrate-binding protein/permease -
KFV43_RS05430 (KFV43_05430) 1020972..1021694 + 723 WP_001829838.1 amino acid ABC transporter ATP-binding protein -
KFV43_RS05435 (KFV43_05435) 1022209..1022346 + 138 WP_064783702.1 hypothetical protein -
KFV43_RS05440 (KFV43_05440) 1022556..1023686 + 1131 WP_001829814.1 tRNA epoxyqueuosine(34) reductase QueG -
KFV43_RS05445 (KFV43_05445) 1023683..1024153 + 471 WP_001829836.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
KFV43_RS05450 (KFV43_05450) 1024311..1024910 + 600 WP_002456349.1 glucosamine-6-phosphate isomerase Antitoxin
KFV43_RS05455 (KFV43_05455) 1024934..1025113 + 180 WP_002468490.1 SAS053 family protein Toxin
KFV43_RS05460 (KFV43_05460) 1025269..1025673 + 405 WP_001829818.1 hypothetical protein -
KFV43_RS05465 (KFV43_05465) 1025858..1027243 + 1386 WP_001829862.1 class II fumarate hydratase -
KFV43_RS05470 (KFV43_05470) 1027604..1028427 - 824 Protein_1034 RluA family pseudouridine synthase -
KFV43_RS05475 (KFV43_05475) 1028586..1029719 + 1134 WP_001829819.1 GAF domain-containing sensor histidine kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1022191..1022346 155


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Glucosamine-6-phosphate isomerase

(130-195)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6963.56 Da        Isoelectric Point: 4.3016

>T200345 WP_002468490.1 NZ_CP073821:1024934-1025113 [Staphylococcus epidermidis]
MANKKDSKLNYHEEENAMVTDLDDLKELGKEMEQISQENDEEKLNQSHDNEVRSDLKKQ

Download         Length: 180 bp

>T200345 NZ_CP097832:c37223-36948 [Shigella flexneri]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG

Antitoxin


Download         Length: 200 a.a.        Molecular weight: 22709.62 Da        Isoelectric Point: 4.9942

>AT200345 WP_002456349.1 NZ_CP073821:1024311-1024910 [Staphylococcus epidermidis]
MAMNFKVFDNSEKVAEYTADILRKQFNNNPTTIAGFHLSKEHAPVFDELKKNVENHTVDFSQINILDYDDNHSYYEALGV
PTGQIYSISYENDAIDFISDKIKTKENKGKLTMQVLTIDENGKLDISVRQGLMEAREIFLVITGANKRDMVEKLYRENGK
TSFEPSDLKAHRMVNVILDKEAAAGLPEDVKEYFTARFA

Download         Length: 600 bp

>AT200345 NZ_CP097832:c37507-37223 [Shigella flexneri]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGTCCTGG
GCGGACAGCCTCAGTACTGACCATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HY44

Antitoxin

Source ID Structure
AlphaFold DB A0A5E9LUD0

References