Detailed information of TA system TA200326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1201408..1201930
• Replicon: chromosome
• Accession: NZ_CP073805
• Organism: Macrococcus canis strain Epi3241-OR

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: KFV08_RS06075
• Protein ID: WP_138071160.1
• Coordinates: 1201408..1201680 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: KFV08_RS06080
• Protein ID: WP_210151726.1
• Coordinates: 1201673..1201930 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFV08_RS06045 (KFV08_06040)	1197492..1197743	+	252	WP_252455606.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
KFV08_RS06050 (KFV08_06045)	1197780..1198226	-	447	WP_252455107.1	GNAT family N-acetyltransferase	-
KFV08_RS06055 (KFV08_06050)	1198306..1199046	+	741	WP_252455108.1	hypothetical protein	-
KFV08_RS06060 (KFV08_06055)	1199056..1199946	+	891	WP_252455109.1	phosphotransferase	-
KFV08_RS06065 (KFV08_06060)	1200132..1200617	+	486	WP_252455110.1	NUDIX domain-containing protein	-
KFV08_RS06070 (KFV08_06065)	1200623..1201390	+	768	WP_252455111.1	hypothetical protein	-
KFV08_RS06075 (KFV08_06070)	1201408..1201680	-	273	WP_138071160.1	Txe/YoeB family addiction module toxin	Toxin
KFV08_RS06080 (KFV08_06075)	1201673..1201930	-	258	WP_210151726.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KFV08_RS06085 (KFV08_06080)	1202219..1202443	+	225	WP_236365521.1	DUF6290 family protein	-
KFV08_RS06090 (KFV08_06085)	1202491..1202706	+	216	WP_252455112.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KFV08_RS06095 (KFV08_06090)	1202817..1203185	+	369	WP_252455113.1	GntR family transcriptional regulator	-
KFV08_RS06100 (KFV08_06095)	1203185..1204285	+	1101	WP_252455114.1	DUF1648 domain-containing protein	-
KFV08_RS06105 (KFV08_06100)	1204413..1204937	+	525	WP_158554239.1	GNAT family N-acetyltransferase	-
KFV08_RS06110 (KFV08_06105)	1205012..1205542	+	531	WP_252455115.1	DinB family protein	-
KFV08_RS06115 (KFV08_06110)	1205553..1206002	+	450	WP_252455116.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10684.42 Da
• Isoelectric Point: 9.7524

>T200326 WP_138071160.1 NZ_CP073805:c1201680-1201408 [Macrococcus canis]
MTKKKILFYSSGFEDYTYWQTNDKTVLKKINKLIKSIMIDGANEGIGKPEKLLGNYTGCYSRRITHEHRLVYKIDDDSIC
ILQCRFHYKR

Nucleotide

• Length: 273 bp

>T200326 NZ_CP097829:c148868-148563 [Shigella flexneri]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9737.75 Da
• Isoelectric Point: 4.0808

>AT200326 WP_210151726.1 NZ_CP073805:c1201930-1201673 [Macrococcus canis]
MKVTTYSNARSNFRKIIDIVNEDSEPYTITTNTHNAVILSEQDYNSMIETLYLQSTPANAERLQKAIDESIDKNNLLQVE
IDLDD

Nucleotide

• Length: 258 bp

>AT200326 NZ_CP097829:c149088-148870 [Shigella flexneri]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA