Detailed information of TA system TA200324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1237930..1238452
• Replicon: chromosome
• Accession: NZ_CP073804
• Organism: Macrococcus canis strain Epi0100-OL

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: KFV07_RS06405
• Protein ID: WP_086042495.1
• Coordinates: 1237930..1238202 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: KFV07_RS06410
• Protein ID: WP_086042496.1
• Coordinates: 1238195..1238452 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFV07_RS06370 (KFV07_06370)	1233784..1234035	+	252	WP_086042489.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
KFV07_RS06375 (KFV07_06375)	1234035..1234295	+	261	WP_210151720.1	Txe/YoeB family addiction module toxin	-
KFV07_RS06380 (KFV07_06380)	1234296..1234745	-	450	WP_254255925.1	GNAT family N-acetyltransferase	-
KFV07_RS06385 (KFV07_06385)	1234825..1235568	+	744	WP_254255926.1	hypothetical protein	-
KFV07_RS06390 (KFV07_06390)	1235578..1236468	+	891	WP_254255927.1	phosphotransferase	-
KFV07_RS06395 (KFV07_06395)	1236654..1237139	+	486	WP_254255928.1	NUDIX domain-containing protein	-
KFV07_RS06400 (KFV07_06400)	1237145..1237912	+	768	WP_254255929.1	hypothetical protein	-
KFV07_RS06405 (KFV07_06405)	1237930..1238202	-	273	WP_086042495.1	Txe/YoeB family addiction module toxin	Toxin
KFV07_RS06410 (KFV07_06410)	1238195..1238452	-	258	WP_086042496.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KFV07_RS06415 (KFV07_06415)	1238723..1239724	+	1002	WP_254255930.1	GNAT family N-acetyltransferase	-
KFV07_RS06420 (KFV07_06420)	1239748..1240242	+	495	WP_254255931.1	isochorismatase family protein	-
KFV07_RS06425 (KFV07_06425)	1240652..1242265	+	1614	WP_254255932.1	ABC-F type ribosomal protection protein	-
KFV07_RS06430 (KFV07_06430)	1242332..1243108	+	777	WP_099578018.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10694.45 Da
• Isoelectric Point: 9.7524

>T200324 WP_086042495.1 NZ_CP073804:c1238202-1237930 [Macrococcus canis]
MPKKKILFYSSGFEDYTYWQTNDKTVLKKINKLIKSIMIDGANEGIGKPEKLLGNYTGCYSRRITHEHRLVYKIEDDSIC
ILQCRFHYKR

Nucleotide

• Length: 273 bp

>T200324 NZ_CP097829:64404-64886 [Shigella flexneri]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTATTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9697.75 Da
• Isoelectric Point: 4.1540

>AT200324 WP_086042496.1 NZ_CP073804:c1238452-1238195 [Macrococcus canis]
MKVTTYSNARSNFRKIIDIVNEDCEPYTITTNTHNAVILSEQDYNSMIETLYLQSTPANAERLQKAIDESTDKNNLLQVE
IDLDA

Nucleotide

• Length: 258 bp

>AT200324 NZ_CP097829:64111-64413 [Shigella flexneri]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA