Detailed information of TA system TA200323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe(toxin)
• Location: 1233784..1234295
• Replicon: chromosome
• Accession: NZ_CP073804
• Organism: Macrococcus canis strain Epi0100-OL

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: KFV07_RS06375
• Protein ID: WP_210151720.1
• Coordinates: 1234035..1234295 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: KFV07_RS06370
• Protein ID: WP_086042489.1
• Coordinates: 1233784..1234035 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFV07_RS06345 (KFV07_06345)	1229381..1230322	+	942	WP_211553181.1	LCP family protein	-
KFV07_RS06350 (KFV07_06350)	1230360..1230548	-	189	WP_086042483.1	2-hydroxymuconate tautomerase	-
KFV07_RS06355 (KFV07_06355)	1230597..1231601	-	1005	WP_211553179.1	cation diffusion facilitator family transporter	-
KFV07_RS06360 (KFV07_06360)	1231763..1232143	+	381	WP_211553177.1	sporulation protein	-
KFV07_RS06365 (KFV07_06365)	1232173..1233084	+	912	WP_210151718.1	hypothetical protein	-
KFV07_RS06370 (KFV07_06370)	1233784..1234035	+	252	WP_086042489.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KFV07_RS06375 (KFV07_06375)	1234035..1234295	+	261	WP_210151720.1	Txe/YoeB family addiction module toxin	Toxin
KFV07_RS06380 (KFV07_06380)	1234296..1234745	-	450	WP_254255925.1	GNAT family N-acetyltransferase	-
KFV07_RS06385 (KFV07_06385)	1234825..1235568	+	744	WP_254255926.1	hypothetical protein	-
KFV07_RS06390 (KFV07_06390)	1235578..1236468	+	891	WP_254255927.1	phosphotransferase	-
KFV07_RS06395 (KFV07_06395)	1236654..1237139	+	486	WP_254255928.1	NUDIX domain-containing protein	-
KFV07_RS06400 (KFV07_06400)	1237145..1237912	+	768	WP_254255929.1	hypothetical protein	-
KFV07_RS06405 (KFV07_06405)	1237930..1238202	-	273	WP_086042495.1	Txe/YoeB family addiction module toxin	-
KFV07_RS06410 (KFV07_06410)	1238195..1238452	-	258	WP_086042496.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-59)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10245.91 Da
• Isoelectric Point: 10.1613

>T200323 WP_210151720.1 NZ_CP073804:1234035-1234295 [Macrococcus canis]
MYKIVLKSSVKKDLKKIKNTLLETNFLKIINQLKENPYNNNHSFEKLIPPIKGFYSRRINVQHRVVYTVNDEDKIVTIYS
AYGHYE

Nucleotide

• Length: 261 bp

>T200323 NZ_CP097829:22546-23031 [Shigella flexneri]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCAGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGAGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9590.65 Da
• Isoelectric Point: 4.0373

>AT200323 WP_086042489.1 NZ_CP073804:1233784-1234035 [Macrococcus canis]
MNTYTPTSARKDFFNIIKSVNEDSKEIYIAPTKIGEKGAVLVGEDDWNAIQETLFLLEKGIAYQIEQRKDEEEIDFDSTW
DNI

Nucleotide

• Length: 252 bp

>AT200323 NZ_CP097829:22274-22558 [Shigella flexneri]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA