Detailed information of TA system TA200318

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1197604..1198126
• Replicon: chromosome
• Accession: NZ_CP073799
• Organism: Macrococcus canis strain Epi0089

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: KFV05_RS06010
• Protein ID: WP_164953490.1
• Coordinates: 1197604..1197876 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: KFV05_RS06015
• Protein ID: WP_210151726.1
• Coordinates: 1197869..1198126 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFV05_RS05980 (KFV05_05985)	1192954..1193205	+	252	WP_133446202.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
KFV05_RS05985 (KFV05_05990)	1193242..1193688	-	447	WP_254246817.1	GNAT family N-acetyltransferase	-
KFV05_RS05990 (KFV05_05995)	1193895..1195036	+	1142	Protein_1183	IS3 family transposase	-
KFV05_RS05995 (KFV05_06000)	1195252..1196142	+	891	WP_254246818.1	phosphotransferase	-
KFV05_RS06000 (KFV05_06005)	1196328..1196813	+	486	WP_252455110.1	NUDIX domain-containing protein	-
KFV05_RS06005 (KFV05_06010)	1196819..1197586	+	768	WP_254245811.1	hypothetical protein	-
KFV05_RS06010 (KFV05_06015)	1197604..1197876	-	273	WP_164953490.1	Txe/YoeB family addiction module toxin	Toxin
KFV05_RS06015 (KFV05_06020)	1197869..1198126	-	258	WP_210151726.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KFV05_RS06020 (KFV05_06025)	1198397..1198933	+	537	WP_254245812.1	GrpB family protein	-
KFV05_RS06025 (KFV05_06030)	1198938..1199387	+	450	WP_233455643.1	GNAT family N-acetyltransferase	-
KFV05_RS06030 (KFV05_06035)	1199389..1199589	+	201	WP_164942134.1	HAD family hydrolase	-
KFV05_RS06035 (KFV05_06040)	1199752..1200219	+	468	WP_254245813.1	hypothetical protein	-
KFV05_RS06040 (KFV05_06045)	1200316..1200684	+	369	WP_099578022.1	GntR family transcriptional regulator	-
KFV05_RS06045 (KFV05_06050)	1200687..1201790	+	1104	WP_254245814.1	DUF1648 domain-containing protein	-
KFV05_RS06050 (KFV05_06055)	1201918..1202442	+	525	WP_254245815.1	GNAT family N-acetyltransferase	-
KFV05_RS06055 (KFV05_06060)	1202517..1203047	+	531	WP_252455115.1	DinB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10698.44 Da
• Isoelectric Point: 9.7524

>T200318 WP_164953490.1 NZ_CP073799:c1197876-1197604 [Macrococcus canis]
MTKKKILFYSSGFEDYTYWQTNDKTVLKKINKLIKSIMIDGANEGIGKPEKLLGNYTGCYSRRITHEHRLVYKIEDDSIC
ILQCRFHYKR

Nucleotide

• Length: 273 bp

>T200318 NZ_CP097828:c3335899-3335669 [Shigella flexneri]
ATGAAGGTATACGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGGCTTTTCGAACTCAGAATTAAAGG
CAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTGCATAGTTTTGTAAAGAAAG
CGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCATGACTGGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9737.75 Da
• Isoelectric Point: 4.0808

>AT200318 WP_210151726.1 NZ_CP073799:c1198126-1197869 [Macrococcus canis]
MKVTTYSNARSNFRKIIDIVNEDSEPYTITTNTHNAVILSEQDYNSMIETLYLQSTPANAERLQKAIDESIDKNNLLQVE
IDLDD

Nucleotide

• Length: 258 bp

>AT200318 NZ_CP097828:c3335679-3335371 [Shigella flexneri]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACACTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA