Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4847630..4848276 Replicon chromosome
Accession NZ_CP073791
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2010_71621

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag KFO61_RS23845 Protein ID WP_002920560.1
Coordinates 4847929..4848276 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag KFO61_RS23840 Protein ID WP_002920557.1
Coordinates 4847630..4847929 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFO61_RS23820 (KFO61_23820) 4843836..4844594 - 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -
KFO61_RS23825 (KFO61_23825) 4844644..4845474 - 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
KFO61_RS23830 (KFO61_23830) 4845525..4845854 - 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
KFO61_RS23835 (KFO61_23835) 4846059..4847567 + 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
KFO61_RS23840 (KFO61_23840) 4847630..4847929 - 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
KFO61_RS23845 (KFO61_23845) 4847929..4848276 - 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KFO61_RS23850 (KFO61_23850) 4848452..4850899 - 2448 WP_002920561.1 glycogen phosphorylase -
KFO61_RS23855 (KFO61_23855) 4850917..4852350 - 1434 WP_002920564.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T200303 WP_002920560.1 NZ_CP073791:c4848276-4847929 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T200303 NZ_CP097808:4149868-4150086 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCTGACCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCCACATCAGTCTGGAAGTTTATCCGCTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT200303 WP_002920557.1 NZ_CP073791:c4847929-4847630 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT200303 NZ_CP097808:4149467-4149841 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References