Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3799673..3800270 Replicon chromosome
Accession NZ_CP073783
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2014_96855

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KFO63_RS18340 Protein ID WP_004142563.1
Coordinates 3799953..3800270 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KFO63_RS18335 Protein ID WP_004142561.1
Coordinates 3799673..3799960 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFO63_RS18305 (KFO63_18320) 3795753..3796001 + 249 WP_002893037.1 DUF1158 domain-containing protein -
KFO63_RS18310 (KFO63_18325) 3796019..3796360 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KFO63_RS18315 (KFO63_18330) 3796391..3797506 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
KFO63_RS18320 (KFO63_18335) 3797686..3798267 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
KFO63_RS18325 (KFO63_18340) 3798267..3798635 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
KFO63_RS18330 (KFO63_18345) 3798755..3799408 + 654 WP_019704893.1 oxygen-insensitive NAD(P)H nitroreductase -
KFO63_RS18335 (KFO63_18350) 3799673..3799960 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KFO63_RS18340 (KFO63_18355) 3799953..3800270 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KFO63_RS18345 (KFO63_18360) 3800455..3801498 - 1044 WP_019704892.1 DUF2157 domain-containing protein -
KFO63_RS18350 (KFO63_18365) 3802168..3803034 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
KFO63_RS18355 (KFO63_18370) 3803143..3804570 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T200259 WP_004142563.1 NZ_CP073783:c3800270-3799953 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T200259 NZ_CP097766:1472496-1472846 [Paenibacillus polymyxa]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTTAGCCCTACCGTGATCGTAGCAGCCATCACAGCACAGATTCAGAAGG
CAAAGCTGCCTACACACGTGGAAATCGACGCGGCGACGCATGGCTTTGATCGTGATTCTGTTGTTCTTCTGGAGCAAATC
CGGACGATCGACAAGCAAAGGCTTACCGACAAAATCACCCATTTGGATGAAGAAACCATGCGCAAAGTGAGCGATTCGCT
ACAGATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT200259 WP_004142561.1 NZ_CP073783:c3799960-3799673 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT200259 NZ_CP097766:1472208-1472489 [Paenibacillus polymyxa]
TTGGCCAATTTGCAGAACACCAAAAGAATAATGATCAGTTTGCCAGATCATCTCTTGCAGGAAGTGGACGGGATCGTAGC
TATGGAGAATTCCAACCGCAGTGAATTTATCAGGCAGGCAATGAAGCTTTATGTAGGTGAACGTAAAAAACGTTACATTC
GTGAAGCTATGCAGCGTGGATACATGGAAATGGCTAAAATTAACCTGACCATGGCATCTGAAGCCTTTCACGCGGAGGAA
GATGCAAACAGCACCTTGGGCCGTACAGTTAGCGGGGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References