Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 308825..309411 Replicon chromosome
Accession NZ_CP073783
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2014_96855

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag KFO63_RS01455 Protein ID WP_002920800.1
Coordinates 309043..309411 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag KFO63_RS01450 Protein ID WP_004174006.1
Coordinates 308825..309046 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFO63_RS01430 (KFO63_01430) 304982..305908 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KFO63_RS01435 (KFO63_01435) 305905..307182 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
KFO63_RS01440 (KFO63_01440) 307179..307946 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KFO63_RS01445 (KFO63_01445) 307948..308661 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
KFO63_RS01450 (KFO63_01450) 308825..309046 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KFO63_RS01455 (KFO63_01455) 309043..309411 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KFO63_RS01460 (KFO63_01460) 309684..311000 + 1317 WP_019704662.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
KFO63_RS01465 (KFO63_01465) 311107..311994 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
KFO63_RS01470 (KFO63_01470) 311991..312836 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
KFO63_RS01475 (KFO63_01475) 312838..313908 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 303811..314645 10834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T200247 WP_002920800.1 NZ_CP073783:309043-309411 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T200247 NZ_CP097726:4087235-4087387 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT200247 WP_004174006.1 NZ_CP073783:308825-309046 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT200247 NZ_CP097726:c4087186-4087129 [Escherichia coli]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References