Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1827055..1827652 Replicon chromosome
Accession NZ_CP073782
Organism Klebsiella pneumoniae strain IRQBAS106c-genome

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KFU55_RS08885 Protein ID WP_004142563.1
Coordinates 1827055..1827372 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KFU55_RS08890 Protein ID WP_004142561.1
Coordinates 1827365..1827652 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU55_RS08870 (1822759) 1822759..1824186 - 1428 WP_004176980.1 MFS transporter -
KFU55_RS08875 (1824295) 1824295..1825161 + 867 WP_048299254.1 helix-turn-helix transcriptional regulator -
KFU55_RS08880 (1825827) 1825827..1826870 + 1044 WP_135707754.1 DUF2157 domain-containing protein -
KFU55_RS08885 (1827055) 1827055..1827372 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KFU55_RS08890 (1827365) 1827365..1827652 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KFU55_RS08895 (1827917) 1827917..1828570 - 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
KFU55_RS08900 (1828690) 1828690..1829058 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
KFU55_RS08905 (1829058) 1829058..1829639 - 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
KFU55_RS08910 (1829819) 1829819..1830934 + 1116 WP_012737592.1 MBL fold metallo-hydrolase -
KFU55_RS08915 (1830965) 1830965..1831306 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KFU55_RS08920 (1831324) 1831324..1831572 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T200228 WP_004142563.1 NZ_CP073782:1827055-1827372 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T200228 NZ_CP097724:c4282437-4282264 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT200228 WP_004142561.1 NZ_CP073782:1827365-1827652 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT200228 NZ_CP097724:c4282761-4282465 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References