Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 32147..32877 Replicon chromosome
Accession NZ_CP073771
Organism Enterobacter hormaechei strain SH19PTE2

Toxin (Protein)


Gene name higB Uniprot ID A0A837FIL2
Locus tag KFO54_RS00155 Protein ID WP_023302825.1
Coordinates 32147..32461 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A837FJX0
Locus tag KFO54_RS00160 Protein ID WP_015570115.1
Coordinates 32461..32877 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFO54_RS00130 (KFO54_00130) 27813..28898 + 1086 Protein_25 cellulase family glycosylhydrolase -
KFO54_RS00135 (KFO54_00135) 28804..29988 - 1185 WP_063159111.1 multidrug efflux MFS transporter EmrD -
KFO54_RS00140 (KFO54_00140) 30164..30997 - 834 WP_048243364.1 DMT family transporter -
KFO54_RS00145 (KFO54_00145) 31060..31506 - 447 WP_211989546.1 GNAT family N-acetyltransferase -
KFO54_RS00150 (KFO54_00150) 31571..31660 - 90 WP_022646551.1 type I toxin-antitoxin system toxin TisB -
KFO54_RS00155 (KFO54_00155) 32147..32461 + 315 WP_023302825.1 type II toxin-antitoxin system HigB family toxin Toxin
KFO54_RS00160 (KFO54_00160) 32461..32877 + 417 WP_015570115.1 helix-turn-helix domain-containing protein Antitoxin
KFO54_RS00165 (KFO54_00165) 33024..33122 + 99 WP_057979964.1 ilvB operon leader peptide IvbL -
KFO54_RS00170 (KFO54_00170) 33352..35040 + 1689 WP_003861056.1 acetolactate synthase large subunit -
KFO54_RS00175 (KFO54_00175) 35044..35331 + 288 WP_003861055.1 acetolactate synthase small subunit -
KFO54_RS00180 (KFO54_00180) 35414..36007 + 594 WP_003861054.1 transcriptional regulator UhpA -
KFO54_RS00185 (KFO54_00185) 36004..37509 + 1506 WP_211989548.1 signal transduction histidine-protein kinase/phosphatase UhpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12415.51 Da        Isoelectric Point: 10.2825

>T200203 WP_023302825.1 NZ_CP073771:32147-32461 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Download         Length: 315 bp

>T200203 NZ_CP097721:3636089-3636367 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15367.95 Da        Isoelectric Point: 5.7670

>AT200203 WP_015570115.1 NZ_CP073771:32461-32877 [Enterobacter hormaechei]
MIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISRYEANRPEIVALRKEMESVPVGI
AVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Download         Length: 417 bp

>AT200203 NZ_CP097721:3635826-3636086 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FIL2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FJX0

References