Detailed information of TA system TA200198

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 22800..23069
• Replicon: plasmid pYUSHP6-2
• Accession: NZ_CP073769
• Organism: Escherichia coli strain SH19PTE6

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KEH54_RS22835
• Protein ID: WP_001372321.1
• Coordinates: 22944..23069 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	22800..22865 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEH54_RS23745	17812..18267	-	456	Protein_26	hypothetical protein	-
KEH54_RS22805	18569..19096	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
KEH54_RS22810	19154..19387	+	234	WP_000006003.1	DUF905 family protein	-
KEH54_RS22815	19448..21412	+	1965	WP_212480488.1	ParB/RepB/Spo0J family partition protein	-
KEH54_RS22820	21481..21915	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
KEH54_RS22825	21912..22674	+	763	Protein_31	plasmid SOS inhibition protein A	-
-	22643..22867	+	225	NuclAT_0	-	-
-	22643..22867	+	225	NuclAT_0	-	-
-	22643..22867	+	225	NuclAT_0	-	-
-	22643..22867	+	225	NuclAT_0	-	-
KEH54_RS22830	22652..22831	-	180	WP_001309233.1	hypothetical protein	-
-	22800..22865	-	66	-	-	Antitoxin
KEH54_RS23750	22853..23002	+	150	Protein_33	plasmid maintenance protein Mok	-
KEH54_RS22835	22944..23069	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KEH54_RS22840	23370..23666	-	297	Protein_35	hypothetical protein	-
KEH54_RS22845	23966..24262	+	297	WP_001272251.1	hypothetical protein	-
KEH54_RS22850	24373..25194	+	822	WP_001234445.1	DUF932 domain-containing protein	-
KEH54_RS22855	25491..26093	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
KEH54_RS22860	26416..26799	+	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KEH54_RS22865	26993..27664	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
KEH54_RS22870	27801..28028	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B)	-	1..67125	67125

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T200198 WP_001372321.1 NZ_CP073769:22944-23069 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T200198 NZ_CP097721:c2441021-2440845 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT200198 NZ_CP073769:c22865-22800 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin