Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3709790..3710331 Replicon chromosome
Accession NZ_CP073768
Organism Escherichia coli strain SH19PTE6

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag KEH54_RS18065 Protein ID WP_000615983.1
Coordinates 3710053..3710331 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag KEH54_RS18060 Protein ID WP_000729703.1
Coordinates 3709790..3710050 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KEH54_RS18040 (3705466) 3705466..3706251 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
KEH54_RS18045 (3706223) 3706223..3707935 + 1713 Protein_3585 flagellar biosynthesis protein FlhA -
KEH54_RS18050 (3708159) 3708159..3708656 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
KEH54_RS18055 (3708832) 3708832..3709580 - 749 Protein_3587 C40 family peptidase -
KEH54_RS18060 (3709790) 3709790..3710050 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KEH54_RS18065 (3710053) 3710053..3710331 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KEH54_RS18070 (3710487) 3710487..3711227 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KEH54_RS18075 (3711198) 3711198..3711965 - 768 WP_000333380.1 class II glutamine amidotransferase -
KEH54_RS18080 (3712171) 3712171..3712749 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T200196 WP_000615983.1 NZ_CP073768:3710053-3710331 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T200196 NZ_CP097721:2269269-2269556 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT200196 WP_000729703.1 NZ_CP073768:3709790-3710050 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT200196 NZ_CP097721:2269030-2269269 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References