Detailed information of TA system TA200195

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3703594..3704288
• Replicon: chromosome
• Accession: NZ_CP073768
• Organism: Escherichia coli strain SH19PTE6

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: KEH54_RS18025
• Protein ID: WP_001263493.1
• Coordinates: 3703594..3703992 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: KEH54_RS18030
• Protein ID: WP_000554757.1
• Coordinates: 3703995..3704288 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEH54_RS17995 (3699153)	3699153..3699611	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KEH54_RS18000 (3699872)	3699872..3701329	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
KEH54_RS18005 (3701386)	3701386..3701907	-	522	Protein_3577	peptide chain release factor H	-
KEH54_RS18010 (3701906)	3701906..3702109	-	204	Protein_3578	RtcB family protein	-
KEH54_RS18015 (3702355)	3702355..3702807	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
KEH54_RS18020 (3702881)	3702881..3703578	+	698	WP_223367350.1	IS1 family transposase	-
KEH54_RS18025 (3703594)	3703594..3703992	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KEH54_RS18030 (3703995)	3703995..3704288	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KEH54_RS18035 (3704340)	3704340..3705395	-	1056	WP_001226164.1	DNA polymerase IV	-
KEH54_RS18040 (3705466)	3705466..3706251	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
KEH54_RS18045 (3706223)	3706223..3707935	+	1713	Protein_3585	flagellar biosynthesis protein FlhA	-
KEH54_RS18050 (3708159)	3708159..3708656	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3703285..3703578	293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T200195 WP_001263493.1 NZ_CP073768:c3703992-3703594 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T200195 NZ_CP097721:1993038-1993141 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT200195 WP_000554757.1 NZ_CP073768:c3704288-3703995 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT200195 NZ_CP097721:c1993179-1992908 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6