Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 672667..673394 Replicon chromosome
Accession NZ_CP073768
Organism Escherichia coli strain SH19PTE6

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KEH54_RS03235 Protein ID WP_000550189.1
Coordinates 672667..672981 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KEH54_RS03240 Protein ID WP_000560266.1
Coordinates 672978..673394 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KEH54_RS03215 (668825) 668825..669811 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
KEH54_RS03220 (669890) 669890..670582 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
KEH54_RS03225 (670659) 670659..671162 - 504 WP_001333820.1 M48 family metallopeptidase -
KEH54_RS03230 (671247) 671247..672383 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KEH54_RS03235 (672667) 672667..672981 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KEH54_RS03240 (672978) 672978..673394 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KEH54_RS03245 (673439) 673439..675457 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KEH54_RS03250 (675783) 675783..678134 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T200179 WP_000550189.1 NZ_CP073768:672667-672981 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T200179 NZ_CP097719:4595577-4595954 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACCACGGCCTCGCGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT200179 WP_000560266.1 NZ_CP073768:672978-673394 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT200179 NZ_CP097719:4595119-4595487 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCTGTATTAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References