Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1426722..1427307 Replicon chromosome
Accession NZ_CP073754
Organism Methylomonas paludis strain S2AM

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag KEF85_RS06520 Protein ID WP_215584242.1
Coordinates 1427026..1427307 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KEF85_RS06515 Protein ID WP_215584240.1
Coordinates 1426722..1427033 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KEF85_RS06500 (KEF85_06505) 1424063..1424449 - 387 WP_215584235.1 type II toxin-antitoxin system VapC family toxin -
KEF85_RS06505 (KEF85_06510) 1424446..1424772 - 327 WP_215584236.1 DUF2281 domain-containing protein -
KEF85_RS06510 (KEF85_06515) 1424785..1426710 - 1926 WP_215584238.1 site-specific DNA-methyltransferase -
KEF85_RS06515 (KEF85_06520) 1426722..1427033 - 312 WP_215584240.1 helix-turn-helix transcriptional regulator Antitoxin
KEF85_RS06520 (KEF85_06525) 1427026..1427307 - 282 WP_215584242.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KEF85_RS06525 (KEF85_06530) 1427388..1428053 - 666 WP_215584243.1 DUF4391 domain-containing protein -
KEF85_RS06530 (KEF85_06535) 1428050..1431322 - 3273 WP_215584245.1 SNF2-related protein -
KEF85_RS06535 (KEF85_06540) 1431579..1431851 - 273 WP_215584253.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(3-88)

Antitoxin

Cro/C1-type helix-turn-helix domain

(46-99)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10363.30 Da        Isoelectric Point: 10.2205

>T200156 WP_215584242.1 NZ_CP073754:c1427307-1427026 [Methylomonas paludis]
VLKLPSGILADYIHLTDLIQHHGTDLRMPHSRAMGDGLFELRPKGPEGIGRVFYCMQLGCTVVILHSFIKKTNTTPVADL
KLAKKRLKEVKNA

Download         Length: 282 bp

>T200156 NZ_CP097717:112902-113207 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11235.77 Da        Isoelectric Point: 7.4007

>AT200156 WP_215584240.1 NZ_CP073754:c1427033-1426722 [Methylomonas paludis]
MHNDEHYQPVPHDAAFRAQLLANPEVQAAFQANAAEYAILDEMLNARKEAGLTQAQVAERMGTKAPAVARLENSLASGKH
SPSIDTLQKYAKALGKKLEVRLV

Download         Length: 312 bp

>AT200156 NZ_CP097717:112682-112900 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References