Detailed information of TA system TA200147

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-YefM
• Location: 2493881..2494383
• Replicon: chromosome
• Accession: NZ_CP073751
• Organism: Halopseudomonas nanhaiensis strain SCS 2-3

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: KEM63_RS11215
• Protein ID: WP_223651678.1
• Coordinates: 2493881..2494144 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: KEM63_RS11220
• Protein ID: WP_150277869.1
• Coordinates: 2494141..2494383 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEM63_RS11190 (KEM63_11135)	2489172..2490551	+	1380	WP_223651672.1	class II fumarate hydratase	-
KEM63_RS11195 (KEM63_11140)	2490745..2492199	+	1455	WP_223651673.1	catalase	-
KEM63_RS11200 (KEM63_11145)	2492326..2492667	-	342	WP_223651674.1	hypothetical protein	-
KEM63_RS11205 (KEM63_11150)	2492709..2493019	-	311	Protein_2204	MerR family DNA-binding protein	-
KEM63_RS11210 (KEM63_11155)	2493136..2493774	-	639	WP_223651676.1	glutathione S-transferase family protein	-
KEM63_RS11215 (KEM63_11160)	2493881..2494144	-	264	WP_223651678.1	Txe/YoeB family addiction module toxin	Toxin
KEM63_RS11220 (KEM63_11165)	2494141..2494383	-	243	WP_150277869.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KEM63_RS11225 (KEM63_11170)	2494648..2494851	-	204	WP_223651680.1	hypothetical protein	-
KEM63_RS11230 (KEM63_11175)	2494960..2495478	-	519	WP_223651682.1	hypothetical protein	-
KEM63_RS11235 (KEM63_11180)	2495497..2496012	-	516	WP_223651685.1	hypothetical protein	-
KEM63_RS11240 (KEM63_11185)	2496449..2496751	-	303	WP_223651687.1	YrhB domain-containing protein	-
KEM63_RS11245 (KEM63_11190)	2496965..2497930	+	966	Protein_2212	integron integrase	-
KEM63_RS11250 (KEM63_11195)	2498142..2499122	-	981	WP_223651688.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-68)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10087.82 Da
• Isoelectric Point: 10.3349

>T200147 WP_223651678.1 NZ_CP073751:c2494144-2493881 [Halopseudomonas nanhaiensis]
VTWKLVYTKQAQKDAKKLASSGLKPKAQELLALIAENPYRKPPPFEKLIGDLAGAYSRRINIQHRLVYQVLEDEKVVKVL
RLWSHYE

Nucleotide

• Length: 264 bp

>T200147 NZ_CP097716:c3985471-3985073 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9074.16 Da
• Isoelectric Point: 4.0533

>AT200147 WP_150277869.1 NZ_CP073751:c2494383-2494141 [Halopseudomonas nanhaiensis]
MTGITATEARSNLYRLIDETAESHQPIVIMGKRNKAVLISEDDWSAIQETLYLLSVPGMRESIREGMDTSVDECDEELNW

Nucleotide

• Length: 243 bp

>AT200147 NZ_CP097716:c3985767-3985474 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA