Detailed information of TA system TA200132

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 7542919..7543619
• Replicon: chromosome
• Accession: NZ_CP073721
• Organism: Dactylosporangium roseum strain NRRL B-16295

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: Drose_RS35430
• Protein ID: WP_260725566.1
• Coordinates: 7543248..7543619 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: Drose_RS35425
• Protein ID: WP_260725565.1
• Coordinates: 7542919..7543251 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Drose_RS35395 (Drose_35135)	7539124..7539432	+	309	WP_260725559.1	calcium-binding protein	-
Drose_RS35400 (Drose_35140)	7539436..7540593	+	1158	WP_260725560.1	hypothetical protein	-
Drose_RS35405 (Drose_35145)	7540851..7541276	-	426	WP_260725561.1	PIN domain nuclease	-
Drose_RS35410 (Drose_35150)	7541273..7541482	-	210	WP_260725562.1	type II toxin-antitoxin system VapB family antitoxin	-
Drose_RS35415 (Drose_35155)	7541588..7542064	+	477	WP_260725563.1	hypothetical protein	-
Drose_RS35420	7542446..7542733	-	288	WP_260725564.1	antitoxin VbhA family protein	-
Drose_RS35425 (Drose_35165)	7542919..7543251	-	333	WP_260725565.1	helix-turn-helix transcriptional regulator	Antitoxin
Drose_RS35430 (Drose_35170)	7543248..7543619	-	372	WP_260725566.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Drose_RS35440	7544324..7544446	-	123	WP_260725567.1	hypothetical protein	-
Drose_RS35445	7544712..7545554	+	843	Protein_7021	YbfB/YjiJ family MFS transporter	-
Drose_RS35450 (Drose_35195)	7545755..7545958	-	204	WP_260725568.1	hypothetical protein	-
Drose_RS35455 (Drose_35205)	7546439..7546924	+	486	WP_260729930.1	GYD domain-containing protein	-
Drose_RS35460 (Drose_35210)	7547109..7547747	+	639	WP_260725569.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (21-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-97)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13853.77 Da
• Isoelectric Point: 4.7352

>T200132 WP_260725566.1 NZ_CP073721:c7543619-7543248 [Dactylosporangium roseum]
MWDVRLHPDVEAWFLELCRVDPITADLVAEAIDVLTEQGPALGRPLVDRLKGSLYQNMKELRPGSAGRSEVRMIFAFDPA
REAIFLVAGDKSGQWQTWYRNAIPLADTRYADHLAALKEEGQS

Nucleotide

• Length: 372 bp

>T200132 NZ_CP097716:c633645-633181 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 11813.35 Da
• Isoelectric Point: 6.3218

>AT200132 WP_260725565.1 NZ_CP073721:c7543251-7542919 [Dactylosporangium roseum]
VTEASNWQDVKAKARAADPTWDSAERVARRQQMREQMLASVSGAQLAEIRKQLGVTQAQLAEATGLTQARISQIENGDAV
GLETLRAYVVGLGGQLDIVARIGNIQLNVA

Nucleotide

• Length: 333 bp

>AT200132 NZ_CP097716:c633980-633645 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA