Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3815220..3815914 Replicon chromosome
Accession NZ_CP073719
Organism Escherichia coli strain CX12

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag J6O05_RS19015 Protein ID WP_001263493.1
Coordinates 3815220..3815618 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID -
Locus tag J6O05_RS19020 Protein ID WP_001399937.1
Coordinates 3815621..3815914 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O05_RS18985 (3810283) 3810283..3810741 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
J6O05_RS18990 (3811002) 3811002..3812459 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
J6O05_RS18995 (3812516) 3812516..3813037 - 522 Protein_3720 peptide chain release factor H -
J6O05_RS19000 (3813033) 3813033..3813239 - 207 Protein_3721 RtcB family protein -
J6O05_RS19005 (3813521) 3813521..3814501 - 981 WP_049109356.1 IS5-like element IS5 family transposase -
J6O05_RS19010 (3814758) 3814758..3815210 - 453 WP_072146325.1 GNAT family N-acetyltransferase -
J6O05_RS19015 (3815220) 3815220..3815618 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
J6O05_RS19020 (3815621) 3815621..3815914 - 294 WP_001399937.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
J6O05_RS19025 (3815966) 3815966..3817021 - 1056 WP_001226161.1 DNA polymerase IV -
J6O05_RS19030 (3817092) 3817092..3817877 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
J6O05_RS19035 (3817849) 3817849..3819561 + 1713 Protein_3728 flagellar biosynthesis protein FlhA -
J6O05_RS19040 (3819879) 3819879..3820376 - 498 WP_000006260.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3813521..3814501 980
- inside Genomic island - gmhA/lpcA / rhs/PAAR / vgrG/tssI / hcp2/tssD2 / tssA / hcp1/tssD1 3780298..3845660 65362


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T200125 WP_001263493.1 NZ_CP073719:c3815618-3815220 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T200125 NZ_CP097713:c42044-41892 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11267.78 Da        Isoelectric Point: 5.2170

>AT200125 WP_001399937.1 NZ_CP073719:c3815914-3815621 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARFEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT200125 NZ_CP097713:42099-42156 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure

References